GENE TARGETING APPROACHES TO MCH AND ENERGY BALANCE

MCH 和能量平衡的基因靶向方法

• 批准号: 6649210
• 负责人: ELEFTHERIA MARATOS-FLIER
• 金额: $ 14.15万
• 依托单位: JOSLIN DIABETES CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6649210
• 关键词: appetite; bioenergetics; dietary lipid; gene targeting; genetic models; genetically modified animals; laboratory mouse; leptin; melanins; melanocyte stimulating hormone; neuroendocrine system; neuropeptide Y; neuropeptides; nutrition related tag; obesity; phenotype

DESCRIPTION: (Adapted from the applicant's abstract) This study proposes to characterize the lean phenotype in MCH knockout mice, and to ascertain the relative contributions of MCH and the two other peptides derived from the MCH gene, N-EI and N-GE. To determine the role of MCH in mediating the orexigenic or appetite inhibiting action of other neuropeptides the effect of appetite regulating peptides in MCH-/-will be assessed. In addition to examining the importance of mediating obesity in single gene models of rodent obesity, MCH-/-mice will be crossbred to leptin deficient (ob/ob) mice and leptin resistant A (agouti) mice. MCH-/- mice will be crossbred to mice with ablations NPY or orexin, other neuropeptides implicated in energy balance. Finally to determine the importance of MCH in mediating obesity in response to environmental agents, the ability of a high fat diet, MSG and GTG to cause obesity will be compared in wild type and MCH-/- animals.

描述：(改编自申请人的摘要)这项研究建议 鉴定MCH基因敲除小鼠的瘦肉表型，并确定 MCH及其衍生的另外两种多肽的相对贡献 基因、N-EI和N-Ge。确定MCH在调节食欲不振中的作用 或抑制食欲的其他神经肽的食欲作用 将对MCH-/-中的调节肽进行评估。除了研究 在单基因啮齿动物肥胖模型中调节肥胖的重要性， MCH-/-小鼠将与瘦素缺乏(ob/ob)小鼠和瘦素杂交 耐药A(刺鼠)小鼠。MCH-/-小鼠将与消融小鼠杂交 神经肽Y或食欲素，其他与能量平衡有关的神经肽。终于到了 确定MCH在调节肥胖反应中的重要性 环境因素、高脂肪饮食的能力、味精和GTG引起 我们将比较野生型和MCH-/-动物的肥胖情况。