PDGF GENE REGULATION BY VITAMINS A & D IN ALVEOLIZATION

维生素 A 对 PDGF 基因的调节

• 批准号: 6527361
• 负责人: David M Kaetzel
• 金额: $ 22.89万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-29 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6527361
• 关键词: cell line; cell proliferation; dexamethasone; fibroblasts; gene expression; gene targeting; genetic promoter element; genetic regulation; genetic transcription; genetically modified animals; growth factor receptors; histogenesis; laboratory rat; lung alveolus; lung development; platelet derived growth factor; respiratory function; retinoids; tissue /cell culture; vitamin D

The investigator proposes to assess the effect of RA, VitD, analogs, and dexamethasone individually or in combination on cell proliferation and expression of PDGFs and their receptors during lung development in vitro (Aim 1) and in vivo (Aim 2). This will include an evaluation of the effect of these treatments on alveolar formation and on lung function (Aim 2). Then, an analysis of the mechanisms regulating PDGF expression will be performed, aiming to characterize cis and trans elements potentially important on mediating transcriptional activation of the PDGF-A chain promoter during lung development (Aims 3 and 4).

研究者建议评估RA、VitD、 类似物， 和地塞米松单独或组合对细胞增殖的影响 PDGFs及其受体在体外肺发育过程中的表达 (Aim 1）和体内（目的2）。这将包括对以下方面的影响进行评价： 这些治疗对肺泡形成和肺功能的影响（目的2）。了一种 将进行调节PDGF表达的机制的分析， 为了表征顺式和反式元件， 肺发育过程中PDGF-A链启动子的转录激活 (Aims第3和第4段）。