Peripheral Blood Monocytes In Myobacterial Infections

单核细胞的CD40、CD80、CD86、CD120b和γ干扰素受体阿尔法链的表达水平正在被表征。这些研究已经确认了两名患有干扰素受体阿尔法链缺陷的患者，这种缺陷的分子水平正在积极进行检查。此外，1例患者还在分子水平上表现出干扰素γ受体β链缺陷的特征。作为这些研究的结果，已经开发了一种细胞内流式细胞术方法来评估STAT蛋白的磷酸化，并初步验证了STAT1和最近的STAT4。细胞表面受体和细胞因子反应水平上的功能状态的结合正被应用于新患者，也被用于评估骨髓移植后的患者。

Monocytes are being characterized for the expression levels of CD40, CD80, CD86, CD120b, and the gamma interferon receptor alpha chain. These studies have identified two patients with gamma interferon receptor alpha chain deficiency, and the molecular level of this defect is being actively examined. In addition, interferon gamma receptor beta chain deficiency has also been characterized in one patient at the molecular level. As a consequence of these studies, an intracellular flow cytometric method for evaluating STAT protein phosphorylation has been developed and validated initially for STAT1 and more recently for STAT4. The combination of cell surface receptor and functional status at the level of cytokine response is being applied to new patients and is also being used to evaluate patients after bone marrow transplantation.