Effect of Diet on Oxidative Stress, NO and Hypertension

饮食对氧化应激、一氧化氮和高血压的影响

基本信息

批准号：
6647161
负责人：
CHRISTIAN K Roberts
金额：
$ 4.99万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-09-30 至 2004-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6647161
关键词：
antioxidants blood chemistry blood pressure carbohydrates diet free radical oxygen hypertension insulin sensitivity /resistance laboratory rat nitric oxide nitric oxide synthase nutrition related tag oxidative stress postdoctoral investigator urinalysis

项目摘要

DESCRIPTION (provided by applicant): The specific aim of this proposal is to investigate potential mechanisms by which a diet high in saturated fat and refined carbohydrates (sucrose) induces insulin resistance and hypertension compared to a diet high in unrefined carbohydrate and low in fat. Hypertension afflicts one quarter of the adult population in the United States and is a hallmark risk factor for myocardial infarction, stroke and congestive heart failure. Although its cause is largely unknown, it is associated with insulin resistance/ hyperinsulinemia, oxidative stress and endothelial dysfunction. The primary hypothesis to be tested in this grant is that a high-fat, refined carbohydrate diet (HFS) increases the production of reactive oxygen/nitrogen species (e.g. ONOO-), contributing to insulin resistance, as well as sequestrating and inactivating NO leading to hypertension. To test this hypothesis, male and female rats will be raised on either the HFS or a low-fat, complex carbohydrate diet (LFCC) and blood pressure will be measured via the tail-cuff and telemetry methods. Isolated arterial segments will be used to test endothelial function. Plasma/urinary measurements will assess NO metabotites, MDA and cGMP levels. Biochemical studies will be. conducted on several tissues, including aorta, heart and kidney to measure eNOS, iNOS, nNOS and SOD proteins, NOS and SOD activities, nitrotyrosine formation and tissue cGMP levels. Studies will also be conducted to investigate the value of dietary modification (switching to an LFCC diet or antioxidant supplementation) and exercise training for controlling hypertension, as well as the role of caloric restriction on the development of hypertension. The results of these studies may provide a mechanism to explain how HFS type diets cause insulin resistance and hypertension, and how a LFCC diet, antioxidant therapy or exercise training aid in the control of insulin resistance and hypertension.

描述（由申请人提供）：该提案的具体目的是调查饮食高饱和脂肪和饮食的潜在机制精制碳水化合物（蔗糖）诱导胰岛素抵抗和高血压与未精制的碳水化合物和低脂肪的饮食相比。高血压折磨在美国的成年人口的四分之一，是心肌梗塞，中风和充血性心脏的标志风险因素失败。尽管其原因在很大程度上未知，但与胰岛素有关抗性/高胰岛素血症，氧化应激和内皮功能障碍。这在这笔赠款中要检验的主要假设是高脂的精制假设碳水化合物饮食（HFS）增加了活性氧/氮的产生物种（例如Onoo-），有助于胰岛素抵抗，以及隔离和灭活不会导致高血压。测试这个假设，将在HFS或低脂上饲养雄性和雌性大鼠，复杂的碳水化合物饮食（LFCC）和血压将通过尾式和遥测方法。孤立的动脉片段将用于测试内皮功能。血浆/尿测量将评估否代谢岩，MDA和CGMP水平。生化研究将是。进行几个组织，包括主动脉，心脏和肾脏，用于测量eNOS，iNOS，nNOS 以及SOD蛋白，NOS和SOD活性，硝基酪氨酸形成和组织 CGMP级别。还将进行研究以研究饮食的价值修改（切换到LFCC饮食或补充抗氧化剂）和控制高血压的运动训练以及热量的作用对高血压发展的限制。这些研究的结果可以提供一种解释HFS型饮食如何引起胰岛素抵抗的机制和高血压，以及LFCC饮食，抗氧化剂治疗或运动训练如何有助于控制胰岛素抵抗和高血压。