Core--analytical biomarkers (xenobiotics) facility

描述(由申请人提供) 分析核心将为这三项中的每一项提供研究支持 中心支持的研究项目。它提供了多样化的阵列 可用于以下方面的分析仪器和专业知识 所有参与该项目的科学家。该核心还提供以下专业知识 为客户提供一次性或常规的分析支持 从亲脂性外源化合物到生物聚合物的各种分子。 可用的技术包括各种层析系统， 分光光度计、质谱仪和免疫化学。编码 从项目I采集的全血和其他组织样本 将如申请书中详细描述的那样转移到核心I。 根据要进行的分析类型，要么是全血 (例如，重金属分析)或血清将被提取以执行 测试如下所示。具体地说，调查人员将提供 对关于已知关联的假设进行检验的分析支持 自闭症的异种生物免疫毒药和神经毒药。特例 将重点关注多卤代化合物的详细分析 有机(例如，多氯联苯、多溴联苯和PDBE)、金属形态(例如，汞， 铅、铜、锌等)和优先杀虫剂怀疑是 在加利福尼亚州，这是神经毒性和环境问题。这个 调查人员还将提供食品的高通量分析， 血清和组织脂类来检验自闭症患者 这种情况可能与饮食中的血脂或血清中的脂质有关 个人资料可以指示自闭症状态。实验室将会 在提供高通量层析方面具有支持功能， 研究项目的物理和免疫化学分析。会的 在改进分析技术方面发挥研究支持作用 并应中心要求开发新的分析技术 调查人员。最后，它将在培训中发挥作用 向项目传达分析核心的能力 科学家和需要动手使用的项目中的培训科学家 分析核心用于项目研究的仪器。

DESCRIPTION (provided by applicant) The Analytical Core will provide research support for each of the three research projects supported by the Center. It provides a diverse array of analytical instruments and expertise that are available for use to all scientists on the project. The core also provides the expertise to offer analytical support on a one time or routine basis for a wide variety of molecules from lipophilic xenobitics to biological polymers. The technologies available include a variety of chromatographic systems, spectrophotometers, mass spectrometry, and immunochemistry. Coded samples of whole blood and other tissue samples collected from Project I will be transferred to Core I as described in detail in the application. Depending on the type of analysis to be performed, either whole blood (e.g., heavy metal profiling) or serum will be extracted to perform tests indicated below. Specifically the investigators will provide the analytical support to test hypotheses regarding the association of known xenobiotic immunotoxicants and neurotoxicants with autism. Particular attention will be focused at detailed analysis of polyhalogenated organic (e.g., PCBs, PBBS, and PDBEs), metals profiling (e.g., mercury, lead, copper, zinc, etc.) and priority pesticides suspected of being neurotoxic and of environmental concern in California. The investigators will also provide a high throughput analysis of food, serum, and tissue lipids to test the hypothesis that the autistic condition may be associated with dietary lipids or that the serum lipid profile may be indicative of the autistic state. The laboratory will have a support function in providing high throughput chromatographic, physical, and immunochemical assays for the research projects. It will have a research support function in improving analytical technologies and developing new analytical technologies requested by center investigators. Finally, it will have a training function in communicating the capabilities of the Analytical Core to project scientists and training scientists from projects who need hands-on use of the Analytical Core's instruments for project research.