NOVEL APPROACHES TO PREVENTING URINARY TRACT INFECTION IN WOMEN

一种单唾液酸神经节苷脂，显示特异性结合 肾盂肾炎大肠从人肾组织中提取并纯化大肠杆菌。 神经节苷脂的完整的一级结构是由一个 1-D和2-D 1H-NMR光谱的组合，电喷雾电离 质谱（ESI-MS）和电喷雾电离/碰撞 全甲基化完整样品的诱导解离MS/MS（ESI-MS/CID-MS） 化合物，并通过GC/EI-MS进行甲基化键分析。 在600 MHz下对800（g的 鞘糖脂，氘交换并溶于DMSO-d 6/2% D2O 在初步积累1-D 1H-NMR谱之后， 聚糖和神经酰胺结构特征的评估是通过 异头质子和其它结构报告基团的比较 与已知的鞘糖脂相似。 一系列标准的二维 然后进行实验（DQF-COSY、TOCSY、NOESY）以分配为 尽可能多的共振，以建立身份和异常形式 所有单糖残基，并建立序列和尽可能多的 通过糖苷间偶极相互作用尽可能地连接位点。 然后通过Hakomori方法将等分试样（100（g））全甲基化， 一半进行ESI-MS和-MS/MS分析（Sciex API-III）， 确认聚糖序列和神经酰胺特征，另一半是 解聚并衍生为部分甲基化的糖醇乙酸酯 用于GC-MS分析的PMAAs（Hewlett-Packard 5890 GC/5970 MSD，DB-5 列）。 通过保留时间和EI对PMAAs进行鉴定 与已知标准相比的碎片模式;这些证实了 所有己糖的特性和键合形式， 神经节苷脂中的2-脱氧-2-乙酰胺基己糖残基。 脂肪酸 通过GC-MS分析由以下物质的甲醇分解获得的甲酯： 神经节苷脂用于确定神经酰胺类型的分布 在这个准备中。 一份描述这项工作的手稿 提交出版。

A monosialosyl ganglioside shown to bind specifically to uropathogenic E. coli was extracted and purified from human kidney. The complete primary structure of the ganglioside was elucidated by a combination of 1- and 2-D 1H-NMR spectroscopy, electrospray ionization mass spectroscopy (ESI-MS) and electrospray ionization/collision induced dissociation MS/MS (ESI-MS/CID-MS) of the permethylated intact compound, and methylation linkage analysis by GC/EI-MS. 1H-NMR spectroscopy at 600 MHz was carried out on 800 (g of glycosphingolipid, deuterium exchanged and dissolved in DMSO-d6/2% D2O. Following preliminary accumulation of a 1-D 1H-NMR spectrum, an assessment of glycan and ceramide structural features was made via comparison of anomeric proton and other structural reporter groups with those of known glycosphingolipids. A standard series of 2-D experiments (DQF-COSY, TOCSY, NOESY) was then performed to assign as many resonances as possible, to establish identity and anom eric form of all monosaccharide residues, and to establish sequence and as many linkage sites as possible via interglycosidic dipolar interactions. An aliquot (100 (g) was then permethylated by the Hakomori method, one half subjected to ESI-MS and -MS/MS analysis (Sciex API-III) to confirm glycan sequence and ceramide features, and the other half was depolymerized and derivatized to partially methylated alditol acetates (PMAAs) for GC-MS analysis (Hewlett-Packard 5890 GC/5970 MSD, DB-5 column). Identification of PMAAs was made by retention times and EI fragmentation patterns compared with known standards; these confirmed both the identity and linkage form of all hexose and 2-deoxy-2-acetamidohexose residues in the ganglioside. Fatty acid analysis by GC-MS of the methyl esters obtained from methanolysis of the ganglioside was used to confirm the distribution of ceramide types in this preparation. A manuscript describing this work has been submitted for publication.