GLYCOSYL COMPOSITION ANALYSIS OF COLLAGENS

胶原蛋白的糖基成分分析

• 批准号: 6653578
• 负责人: STEVEN B LEVERY
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653578
• 关键词: biological products; biomedical resource; carbohydrates; nuclear magnetic resonance spectroscopy; technology /technique

NMR analysis was performed on a Varian Inova 600-MHz spectrometer. The sample was deuterium-exchanged by lyophilization from D2O and dissolved in 0.5 mL D2O. The 1H-NMR 1-D and 2-D TOCSY (total correlation spectroscopy) spectra were acquired at 25(C. Proton chemical shifts were measured relative to an internal acetone standard ((=2.225 ppm). 1H-NMR data were compared with those of a previous sample having the structure proposed for this sample. The data confirmed an identical structure for the major component of this sample, but also suggested the presence of a truncated component.

NMR 分析在 Varian Inova 600-MHz 光谱仪上进行。 样品通过冻干从 D2O 中进行氘交换， 溶于 0.5 mL D2O。 1H-NMR 1-D 和 2-D TOCSY（总 相关光谱）光谱在 25（C.质子 相对于丙酮内标测量化学位移 ((=2.225 ppm)。1H-NMR数据与之前的数据进行了比较 具有为此样品建议的结构的样品。 数据 确认了该结构的主要组成部分具有相同的结构 样本，但也表明存在截断的组件。