NMR ANALYSIS OF OLIGOSACCHARIDES

通过对样品进行单糖组成分析 甲基糖苷三甲基硅基衍生物的制备 其次是气相色谱(GC)和组合气体 色质联用(GC-MS)分析。TMS 样品经3M甲醇裂解制得甲基苷 氨基糖与吡啶和醋酸的HCl，Re-N-乙酰化反应 甲醇中的酸酐，以及与三甲基硅烷的全O-三甲基硅化反应。气相色谱 在HP5890色谱柱上进行了TMS甲基糖苷的分析 采用DB1熔融石英毛细管柱。GC-MS分析表明， 使用连接到5970 MSD的HP 5890 GC执行。 通过比较单糖的保留时间来鉴定单糖 符合标准，这些碳水化合物的特性是 通过它们的质谱图进行了鉴定。

The samples were analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl, re-N-acetylation of amino sugars with pyridine and acetic anhydride in methanol, and per-O-trimethylsilylation with Tri-Sil. GC analysis of the TMS methylglycosides was performed on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP 5890 GC interfaced to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.