NMR ANALYSIS OF GLYCOCONJUGATE

糖复合物的 NMR 分析

• 批准号: 6653576
• 负责人: STEVEN B LEVERY
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653576
• 关键词: biological products; biomedical resource; carbohydrates; drug /agent; nuclear magnetic resonance spectroscopy; technology /technique

Biosynthetic oligosaccharide samples were submitted for confirmation of their structure and generation of standard 1H- and 13C-NMR spectra for company files. The samples were exchanged repeatedly in D2O and subjected to 1H- and 13C-NMR analysis at 500 and 125.77 MHz, respectively. 1-D spectra were acquired on a Bruker AMX-500 NMR instrument at 25(C. Chemical shifts were assigned relative to acetone, and the oligosaccharide sequences, anomeric configurations, and linkages were assigned by comparison with previously published spectra of synthetic oligosaccharides of identical structure.

生物合成低聚糖样品提交给 确认其结构并生成标准1H-和 13 C-NMR光谱供公司存档。 样本被交换了 在D2 O中重复，并在500 ℃下进行1H-和13 C-NMR分析， 125.77 MHz。 1-D光谱在Bruker AMX-500核磁共振仪在25（C。 化学位移进行了归属 相对于丙酮，和寡糖序列，异头 配置，并通过与 先前发表的合成寡糖的光谱 相同的结构。