NMR ANALYSIS OF GLYCOCONJUGATE

糖复合物的 NMR 分析

• 批准号: 6653586
• 负责人: STEVEN B LEVERY
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653586
• 关键词: bacteria; biological products; biomedical resource; carbohydrates; spectrometry

The sample was analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl, re-N-acetylation of amino sugars with pyridine and acetic anhydride in methanol, and per-O-trimethylsilylation with Tri-Sil. GC analysis of the TMS methylglycosides was performed on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP5890 GC interfaced to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

通过以下方法分析样品的单糖组成： 制备甲基糖苷的三甲基硅基衍生物， 然后进行气相色谱（GC）和合并的气体 色谱/质谱（GC-MS）分析。 TMS 通过在3 M甲醇中甲醇分解从样品制备甲基糖苷 HCl，氨基糖与吡啶和乙酸的再-N-乙酰化 在甲醇中的酸酐，和用Tri-Sil的全-O-三甲基甲硅烷基化。 GC TMS甲基糖苷的分析在HP 5890 GC上进行 使用DB 1熔融石英毛细管柱。 GC-MS分析 使用与5970 MSD连接的HP 5890 GC进行。 单糖通过它们的保留时间进行鉴定， 标准，这些碳水化合物的特征是 通过它们的质谱进行鉴定