NMR ANALYSIS OF AN OLIGOSACCHARIDE

低聚糖的 NMR 分析

• 批准号: 6653580
• 负责人: STEVEN B LEVERY
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653580
• 关键词: biological products; biomedical resource; carbohydrates; nuclear magnetic resonance spectroscopy; technology /technique

NMR analysis was performed on a Bruker DRX-600 spectrometer. The sample was deuterium-exchanged by lyophilization from D2O and dissolved in 0.5 mL D2O. The 1H-NMR 1-D spectrum was obtained at 25(C using presaturation of the residual HOD signal. Chemical shifts were measured relative to an internal acetone standard ((=2.225 ppm). Based on the 1-D spectrum, it was established that the mixture contained at least four oligosaccharide components, three of which are of known structure.

在Bruker DRX-600光谱仪上进行NMR分析。 的 通过从D2 O冻干对样品进行氘交换， 溶于0.5 mL D2 O中。 在25 ℃下获得1H-NMR 1-D光谱 使用残余HOD信号的预饱和。 化学位移 相对于丙酮内标（（=2.225 ppm））测量。 基于一维光谱，确定混合物 含有至少四种寡糖成分，其中三种是 已知的结构。