GLYCOSYL COMPOSITION OF COLLAGENS

在Varian Inova 600-MHz光谱仪上进行NMR分析。 样品通过从D2 O冻干进行氘交换， 溶于0.5 mL D2 O中。 1H-NMR一维和二维TOCSY（总 相关光谱法）、13 C-1H-HSQC（杂原子单量子 相关性），和-HMBC（杂原子多重键相关性） 在25 ℃下获得光谱。 测量质子化学位移 相对于丙酮内标。 一维1H-NMR谱数据 与先前发表的寡糖相当 片段的结构与该样品的建议结构相同。 的 单糖序列和连接结构通过a 1H-1H-同系物（TOCSY）和13 C-1H-杂环化合物系列 相关实验（HSQC，HMBC）。

NMR analysis was performed on a Varian Inova 600-MHz spectrometer. The sample was deuterium-exchanged by lyophilization from D2O and dissolved in 0.5 mL D2O. The 1H-NMR 1-D and 2-D TOCSY (total correlation spectroscopy), 13C-1H-HSQC (heteronuclear single quantum correlation), and -HMBC (heteronuclear multiple-bond correlation) spectra were acquired at 25(C. Proton chemical shifts were measured relative to internal acetone standard. The 1-D 1H-NMR spectral data were comparable to those published previously for an oligosaccharide fragment identical in structure to that proposed for this sample. The monosaccharide sequence and linkage structure were confirmed by a series of 1H-1H-homonuclear (TOCSY) and 13C-1H-heteronuclear correlation experiments (HSQC, HMBC).