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HEAT LABILE ENTEROTOXIN FROM E COLI

HEAT LABILE ENTEROTOXIN FROM E COLI
来自大肠杆菌的不耐热肠毒素
批准号:
6658530
负责人:
ETHAN A MERRITT
金额:
$14.32万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-03-01 至 2003-02-28

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中文摘要
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英文摘要
Synchrotron radiation is needed to collect higher resolution data from the LT-IIb holotoxin, both native crystals and soaked or co-crystallized with receptor analogues. If the MR structure solution has not succeeded by then we will use bromine-substituted sugars as receptor analogues, which should yield five Br sites for SIR/SAS phasing. We have constructed several active site mutants of LT, in addition to those previously crystallized. Experience to date is that these mutants produce small, radiation-sensitive, crystals which will require synchrotron radiation to produce adequate data quality.
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