HEAT LABILE ENTEROTOXIN FROM E COLI
HEAT LABILE ENTEROTOXIN FROM E COLI
批准号:
6658530
负责人:
ETHAN A MERRITT
金额:
$14.32万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-03-01 至 2003-02-28
中文摘要
点击翻译按钮获取中文摘要
英文摘要
Synchrotron radiation is needed to collect higher resolution data
from the LT-IIb holotoxin, both native crystals and soaked or
co-crystallized with receptor analogues. If the MR structure solution
has not succeeded by then we will use bromine-substituted sugars as
receptor analogues, which should yield five Br sites for SIR/SAS
phasing. We have constructed several active site mutants of LT, in
addition to those previously crystallized. Experience to date is that
these mutants produce small, radiation-sensitive, crystals which will
require synchrotron radiation to produce adequate data quality.
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