FE XAS L2,3 EDGE STUDIES OF NON HEME IRON ENZYMES

我们建议研究的几何和电子结构的 使用Fe L边X射线的非血红素单核和双核铁酶 吸收光谱学 目前在铁K边的研究是有限的 实验的某些特征。 1s.3d转换是 电偶极禁戒和Fe K边的能量分辨 与d轨道分裂的数量级相同， 配体场 相反，在金属L2处，3边2p.3d跃迁 是电偶极允许的，提供能量的直接探测 d轨道的能级和布居。 铁的系统研究 具有不同连接构型的模型化合物的L边光谱 和氧化态将被用来建立理解的基础 这将适用于单核和双核中的活性位点， 非血红素铁酶

We propose to study the geometric and electronic structure of non-heme mononuclear and dinuclear iron enzymes using Fe L-edge X-ray absorption spectroscopy. Current studies at the Fe K-edge are limited by certain characteristics of the experiment. The 1s.3d transition is electric dipole forbidden and the energy resolution at the Fe K-edge is of the same order of magnitude as splittings of the d-orbitals by a ligand field. In contrast, at the metal L2,3-edges 2p.3d transitions are electric dipole allowed, providing a direct probe of the energy levels and populations of the d-orbitals. A systematic study of Fe L-edge spectra of model compounds with varying ligation, geometries and oxidation states will be used to develop a basis of understanding which will be applied to the active sites in mononuclear and dinuclear non-heme iron enzymes.