MAGNETIC RESONANCE SPECTROSCOPY REVEALS NEURONAL DAMAGE IN SIV INFECTED MACAQUES

磁共振波谱揭示 SIV 感染猕猴的神经元损伤

• 批准号: 6591327
• 负责人: ANDREW A LACKNER
• 金额: $ 11.11万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6591327
• 关键词: AIDS; Mammalia; bioimaging /biomedical imaging; communicable diseases; immunology; inflammation; lymphatic system; nervous system; nuclear magnetic resonance spectroscopy; technology /technique; virus

Infection with human immunodeficiency virus (HIV) commonly results in neurologic disease termed the AIDS dementia complex (ADC) Neuronal loss and injury have been found in the HIV-infected brain, but the underlying mechanisms are poorly understood The simian immunodeficiency virus (SIV) infected macaque is an excellent model for HIV infection, but neuronal loss has not been demonstrated To determine whether neuronal damage occurs in the SIV-infected macaque we quantified the neuronal marker n-acetylaspartate (NAA) using proton magnetic resonance spectroscopy in brain extracts of control and SIV infected macaques and correlated these findings with histologic analyses We found reduced NAA in the SIV-infected animals compared to controls (2 94+1 37 versus 6 21+1 73 mols/g wet weight; p=0 004) A significant decrease in NAA was also found in SIV-infected animals sacrificed in the acute stages of infection We conclude that SIV infection of rhesus macaques re sults in neuronal damage that is demonstrable soon after infection Publications Tracey I, Lane J, Chang I, Navia B, Lackner A, Gonzalez RG 1H-MRS reveals neuronal injury in simian immunodeficiency virus macaque model Journal of Acquired Immune Deficiency Syndrome Human Retroviruses 15:21-27, 1997 Cheng LL, Ma MJ, Becerra L, Ptak T, Tracey I, Lackner A, Gonzalez RG Quantitative neuropathology by high resolution magic angle spinning proton magnetic resonance spectroscopy PNAS, 94:6408-6413, 1997

人类免疫缺陷病毒（HIV）的感染通常结果 在神经疾病中，称为艾滋病痴呆症复合物（ADC）神经元 在感染HIV的大脑中发现了损失和受伤，但是 基本机制对猿猴的理解很差 免疫缺陷病毒（SIV）感染猕猴是一个极好的模型 对于HIV感染，但尚未证明神经元丧失 确定神经元损伤是否发生在SIV感染的猕猴中 我们使用质子量化了神经元标记N-乙酰天冬氨酸（NAA） 对照和SIV的脑提取物中的磁共振光谱 感染猕猴并将这些发现与组织学相关联 分析我们发现，与SIV感染的动物相比， 对照（2 94+1 37对6 21+1 73 mols/g湿重； p = 0 004）a 在SIV感染的动物中也发现了NAA的显着降低 在感染的急性阶段被牺牲，我们得出结论，SIV 猕猴感染神经元损害，即 感染出版物在Tracey I，Lane J，Chang后不久证明 我，Navia B，Lackner A，Gonzalez RG 1H-MRS揭示了神经元损伤 猿猴免疫缺陷病毒猕猴模型杂志获得的免疫 缺乏综合症人类逆转录病毒15：21-27，1997 Cheng LL，Ma MJ， Becerra L，Ptak T，Tracey I，Lackner A，Gonzalez RG定量 高分辨率的神经病理学魔术角旋转质子磁 共振光谱PNAS，94：6408-6413，1997