Microbiologic And Cytologic Results For Lavages

灌洗液的微生物学和细胞学结果

• 批准号: 6542079
• 负责人: Frank G Witebsky
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6542079
• 关键词: Fungi; Mycobacterium tuberculosis; Pneumocystis carinii; analytical method; clinical research; communicable disease diagnosis; cytology; diagnosis design /evaluation; diagnosis quality /standard; diagnostic respiratory lavage; diagnostic tests; human data; microbiology; microorganism classification; respiratory disorder; statistics /biometry

Bronchoalveolar lavage specimens are usually split between cytology and microbiology laboratory analysis. Because different methodologies are used by these two laboratories in the work-up of these specimens, we thought it would be useful to review the results obtained on these specimens by each laboratory. Such a review might help define the relative sensitivities of the different procedures used, suggest areas of redundancy that might be candidates for elimination, and help identify the procedures most likely to produce clinically significant results. Results from the data analyzed thus far indicate that cytology preparations are more sensitive for the direct detection of significant fungal pathogens than the smears prepared in microbiology, presumably because of the larger volume of material used for preparation of smears in cytology. The data for approximately 7 years have been collected and partially analyzed to assess the relative sensitivities of the procedures performed in the two laboratories not only for fungi but also for the detection of other pathogens such as Mycobacterium tuberculosis and Pneumocystis carinii. Further analysis of the data has been temporarily postponed to deal with more pressing projects; we hope to complete the analysis within the next year, perhaps with the inclusion of more recent data.

支气管肺泡灌洗标本通常分为细胞学和微生物学实验室分析。由于这两个实验室在对这些标本的研究中使用了不同的方法，我们认为审查每个实验室对这些标本所获得的结果将是有用的。这样的审查可能有助于确定所使用的不同程序的相对敏感性，建议可能被淘汰的冗余领域，并帮助确定最有可能产生临床显著结果的程序。到目前为止分析的数据结果表明，细胞学制剂比微生物学涂片更敏感地直接检测重要的真菌病原体，这可能是因为细胞学中用于制备涂片的材料体积更大。收集了大约7年的数据，并进行了部分分析，以评估这两个实验室进行的程序的相对敏感性，不仅对真菌，而且对其他病原体，如结核分枝杆菌和卡氏肺孢子虫的检测。对数据的进一步分析已被暂时推迟，以处理更紧迫的项目；我们希望在明年内完成分析，或许还会纳入更新的数据。