Susceptibility Testing Procedures for Rapidly Growing Mycobacteria and Nocardia

没有标准化的药敏试验程序 存在于快速生长的分枝杆菌或诺卡氏菌物种。几个 已经建议使用不同的程序，包括肉汤 微量稀释和Etest(由AB BioDisk，卡尔弗制造 加利福尼亚州城市)。我们正在比较两种微量稀释程序 (JustOne，由Radieter America，Inc.，Westlake制造， 哦)和Etest程序，因为它们的可比性和 可重复性，同时查看多个其他 变量，如接种量、培养时间和 测试介质。Just One条带很有用，因为它们允许 选择可能符合以下条件的个别抗菌剂的稀释 尤其与临床相关，而不是使用整个阵列 在通常的微量稀释板格式中发现的试剂。我们希望 能够选择一种能够提供快速和 结果是可重现的，而且在技术上不会要求太高 不适合在常规诊断实验室使用。工作至今 指示Etest生成的结果很难 我们打算将我们的进一步努力集中在 微量稀释程序。我们还参与了一项 多机构协作研究，以确定跨和 Etest和微量稀释液的机构内重复性 快速生长细菌药敏试验程序 分枝杆菌。已经收集了机构间的结果并 目前正在进行分析。

No standardized susceptibility testing procedures exist for rapidly growing mycobacteria or Nocardia species. Several different procedures have been suggested for use, including broth microdilution and the Etest (manufactured by AB Biodisk, Culver City, CA). We are comparing both a microdilution procedure (JustOne, manufactured by Radiometer America, Inc., Westlake, OH) and the Etest procedure, for their comparability and reproducibility, looking simultaneously at a number of other variables such as inoculum concentration, incubation time, and testing medium.The JustOne strips are useful because they allow selection of dilutions of individual antimicrobial agents that may be particularly relevant clinically, rather than the use of a whole array of agents as found in the usual microdilution plate format. We hope to be able to select a procedure that will provide rapid and reproducible results, and not be so technically demanding as to be unsuitable for use in the routine diagnostic laboratory. Work to date indicates that the Etest produces results that are difficult to interpret, and we intend to concentrate our further efforts on the microdilution procedure. We have also been involved in a multi-institution collaborative study to determine the inter- and intra-institution reproducibility of both the Etest and a microdilution procedure for susceptibility testing of rapidly growing mycobacteria. The inter-institution results have been collected and are currently being analyzed.