USE OF PCR AND RFLP ANALYSIS FOR IDENTIFICATION OF MYCOBACTERIA AND NOCARDIA

使用 PCR 和 RFLP 分析鉴定分枝杆菌和诺卡氏菌

• 批准号: 6289463
• 负责人: Frank G Witebsky
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6289463
• 关键词: Actinomycetales; Mycobacterium; bacterial genetics; genome; microorganism classification; polymerase chain reaction; restriction fragment length polymorphism

Polymerase chain reaction amplification of a portion of the genome of both rapidly growing mycobacteria and nocardia, followed by restriction fragment length polymorphism analysis of the amplification products, is being evaluated to assess the utility of these procedures for use in the diagnostic laboratory. The technique has already proven useful in providing preliminary identification of these organisms within a few days of organ-ism isolation, as compared with the month or more required for conventional identification based on biochemical testing. In addition, these molecular procedures seem generally to allow more accurate discrimination among species and subspecies than is possible with biochemical testing. Our work with a portion of the genome for 16S ribosomal RNA has suggested the existence of a hitherto unrecognized Nocardia species. A manuscript describing our methodology and summarizing our findings to date will be submitted for publication. Further studies will be performed on the organisms which may represent new species in the genus Nocardia.

对快速生长的分枝杆菌和诺卡菌基因组的一部分进行聚合酶链反应扩增，然后对扩增产物进行限制性片段长度多态性分析，以评估这些方法在诊断实验室中的实用性。与基于生化测试的常规鉴定所需的一个月或更长时间相比，该技术已被证明可以在生物体分离后的几天内对这些生物体进行初步鉴定。此外，与生物化学测试相比，这些分子程序似乎通常可以更准确地区分物种和亚种。我们对16S核糖体RNA部分基因组的研究表明，存在一种迄今未被认识的诺卡菌物种。一份描述我们的方法和总结我们迄今为止的发现的手稿将被提交出版。将对可能代表诺卡菌属新物种的生物进行进一步研究。