Signal processing in the vestibular nuclei

前庭核的信号处理

• 批准号: 6626290
• 负责人: MICHAEL H ROWE
• 金额: $ 7.25万
• 依托单位: OHIO UNIVERSITY ATHENS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-19 至 2005-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626290
• 关键词: Chelonia; afferent nerve; biological signal transduction; brain stem; electrophysiology; electrostimulus; method development; neural information processing; neural transmission; single cell analysis; stimulus /response; synapses; tissue /cell preparation; vestibular nuclei

DESCRIPTION (provided by applicant): Over the past 40 years, considerable progress has been made in understanding mechanisms of vestibular function. One major advance has been the recognition that vestibular primary afferents are structurally and physiologically heterogeneous; unfortunately, the functional significance of this diversity remains unclear. A central issue is the fate of afferent signals when they reach the CNS: how do different afferents modulate the activity of central vestibular neurons, and how do these effects depend on the nature of the stimulus? I propose to address this question through simultaneous extracellular recordings from (1) single vestibular nucleus neurons and (2) their eighth nerve afferents in an in vitro preparation of the turtle brainstem. Dynamic afferent stimulation will be provided by sinusoidally modulated currents delivered to the posterior canal. This approach will allow me to: 1) quantify the contribution of individual vestibular afferents to the discharge of secondary neurons; 2) quantify patterns of convergence of vestibular afferents with different functional properties onto individual secondary neurons; 3) quantify the dynamics of signal transmission across the synapse and any changes that occur as a function of stimulus properties. This R21 application seeks funding to establish the feasibility of my dual recording preparation and to conduct pilot studies on synaptic transmission at the first central vestibular synapse. Once these aims are accomplished I will be positioned to pursue my long-term objective: to learn how synaptic transmission between afferents and vestibular nucleus neurons varies as a function of afferent type and the nature of the afferent stimulus.

描述（由申请人提供）：在过去的40年中，大量 在理解前庭功能的机制方面取得了进展。一 主要的进步是认识到前庭主要传入是 在结构和生理上异质上；不幸的是，功能 这种多样性的意义尚不清楚。一个核心问题是命运 传入信号到达CNS时：不同的传入如何调制 中央前庭神经元的活性，这些影响如何取决于 刺激的本质？ 我建议通过同时进行细胞外记录来解决这个问题 来自（1）单个前庭核神经元和（2）其第八神经传入 在体外制备乌龟脑干。动态传入刺激 将由鼻曲线调制电流提供 运河。这种方法将允许我：1）量化 单个前庭传入次级神经元的传入； 2） 量化前庭传入的收敛模式不同 功能特性在单个次级神经元上； 3）量化 信号传输跨突触的动力学以及发生的任何变化 刺激特性的函数。 此R21应用程序寻求资金来确定我的双重的可行性 记录准备并进行有关突触传播的试验研究 第一个中央前庭突触。一旦实现了这些目标，我就会 有能力追求我的长期目标：了解突触传播 传入和前庭核神经元之间 传入类型和传入刺激的性质。