Signal processing in the vestibular nuclei

前庭核的信号处理

• 批准号: 6626290
• 负责人: MICHAEL H ROWE
• 金额: $ 7.25万
• 依托单位: OHIO UNIVERSITY ATHENS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-19 至 2005-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626290
• 关键词: Chelonia; afferent nerve; biological signal transduction; brain stem; electrophysiology; electrostimulus; method development; neural information processing; neural transmission; single cell analysis; stimulus /response; synapses; tissue /cell preparation; vestibular nuclei

DESCRIPTION (provided by applicant): Over the past 40 years, considerable progress has been made in understanding mechanisms of vestibular function. One major advance has been the recognition that vestibular primary afferents are structurally and physiologically heterogeneous; unfortunately, the functional significance of this diversity remains unclear. A central issue is the fate of afferent signals when they reach the CNS: how do different afferents modulate the activity of central vestibular neurons, and how do these effects depend on the nature of the stimulus? I propose to address this question through simultaneous extracellular recordings from (1) single vestibular nucleus neurons and (2) their eighth nerve afferents in an in vitro preparation of the turtle brainstem. Dynamic afferent stimulation will be provided by sinusoidally modulated currents delivered to the posterior canal. This approach will allow me to: 1) quantify the contribution of individual vestibular afferents to the discharge of secondary neurons; 2) quantify patterns of convergence of vestibular afferents with different functional properties onto individual secondary neurons; 3) quantify the dynamics of signal transmission across the synapse and any changes that occur as a function of stimulus properties. This R21 application seeks funding to establish the feasibility of my dual recording preparation and to conduct pilot studies on synaptic transmission at the first central vestibular synapse. Once these aims are accomplished I will be positioned to pursue my long-term objective: to learn how synaptic transmission between afferents and vestibular nucleus neurons varies as a function of afferent type and the nature of the afferent stimulus.

描述（由申请人提供）：在过去的40年里，相当多的 在理解前庭功能的机制方面已经取得了进展。一 主要的进展是认识到前庭初级传入神经是 结构和生理异质性;不幸的是， 这种多样性的意义尚不清楚。一个核心问题是 传入信号到达中枢神经系统时：不同的传入信号如何调节 中枢前庭神经元的活动，以及这些影响如何依赖于 刺激的性质？ 我建议通过同步细胞外记录来解决这个问题 来自（1）单个前庭核神经元和（2）它们的第八神经传入 在海龟脑干的体外制备中。动态传入刺激 将由输送到后部的正弦调制电流提供 运河这种方法将允许我：1）量化的贡献， 次级神经元放电的个体前庭传入; 2） 量化前庭传入的会聚模式， 功能特性到单个次级神经元上; 3）量化 突触上信号传输的动力学以及 刺激属性的函数。 这个R21申请寻求资金，以建立我的双 记录准备和进行突触传递的初步研究， 第一个中央前庭突触一旦这些目标实现，我将 我的长期目标是：了解突触传递是如何 传入神经和前庭核神经元之间的关系随着 传入类型和传入刺激的性质。