Viral Vector Core

病毒载体核心

• 批准号: 6847311
• 负责人: Lester William Cashdollar
• 金额: $ 7.07万
• 依托单位: VERSITI WISCONSIN, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6847311
• 关键词: Adenoviridae; Orthomyxoviridae; West Nile virus; biomedical facility; biotechnology; cellular immunity; cooperative study; cytotoxic T lymphocyte; gene expression; helper T lymphocyte; immune response; immunologic memory; influenza; recombinant virus; transfection /expression vector; vaccinia virus; virus genetics

The vector core laboratory at the Blood Research Institute will provide the viral vectors required to complete the specific aims of the projects described in this application. The proposed research requires efficient delivery and expression of influenza virus genes in both cell culture and mouse model systems. The vector core will clone individual influenza virus genes, subgenomic fragments and mutated genes into shuttle vectors for both vaccinia virus and adenovirus-based viral vector systems. The core lab will use standard methods to mediate recombination of the shuttle vectors into the genome of either adenovirus or vaccinia virus to create a recombinant virus vector. The Core Lab personnel will work with the investigators to select the recombinant virus isolates that best suits their needs. The core lab will then amplify and purify the virus stock and provide a virus titer for the labs requesting the vector be constructed. The vector core lab will therefore function to best serve the needs of investigators by providing them with a powerful and necessary tool for their research programs without the need for them to become experts in the creation of viral vectors.

血液研究所的载体核心实验室将提供完成本申请中描述的项目的特定目标所需的病毒载体。拟议的研究需要在细胞培养物和小鼠模型系统中有效传递和表达流感病毒基因。该载体核心将把单个流感病毒基因、亚基因组片段和突变基因克隆到痘苗病毒和基于腺病毒的病毒载体系统的穿梭载体中。核心实验室将使用标准方法介导穿梭载体重组到腺病毒或痘苗病毒的基因组中，以创建重组病毒载体。核心实验室人员将与研究人员合作，选择最适合他们需求的重组病毒分离株。然后，核心实验室将扩增和纯化病毒库存，并为请求构建载体的实验室提供病毒滴度。因此，载体核心实验室将通过为研究人员的研究项目提供强大且必要的工具来最好地满足研究人员的需求，而无需他们成为病毒载体创建的专家。