Capillary DNA Sequencer/Genetic Analyzer

描述(由申请者提供)：威斯康星州东南部血液中心血液研究所(BRI)对血液相关研究有既定的、长期的承诺。超过15名主要研究人员领导心血管生物学、免疫学、免疫血液学、免疫调节、出血和血栓控制以及输血医学等领域的研究项目。分子生物学、蛋白质化学、杂交瘤、流式细胞术、生物物理学、组织学等六大核心实验室为一带一路实验室提供研究服务。经验丰富的技术人员负责核心实验室的日常运营，并提供调查人员要求的服务。每个核心实验室都有一名科学顾问，他是一名具有所提供技术和服务经验的一带一路调查员。核心实验室经理负责监督这些支持实验室的运行。DNA测序是分子生物学核心实验室提供的一项服务。为了满足一带一路研究人员的需求，我们正在申请资金购买ABI Prism 3100遗传分析仪。由九名NIH资助的研究人员和三名新的研究人员发起的项目需要这个设施来实现他们的研究目标。这些项目的题目是：罕见的触发新生儿全免疫性血小板减少症的血小板特异性AIIo抗原的鉴定；模拟药物依赖抗体行为的重组Fab片段的表征；血小板A和B血型抗原表达的分子基础；vWAgll的结构和功能；vWF改变的分子和细胞影响；GPIB在血栓形成机制中的作用；冠状动脉血栓形成的综合方法；内皮细胞分泌颗粒的蛋白质分选；T细胞对血小板AIIa抗原的反应分析；通过突变研究人类白细胞抗原-DR多态的功能；利用TK表达的T细胞调节GVH/GL；PECAM-1对细胞黏附的分子调控要求的表征；小鼠模型的建立，以研究整合素结构性激活的体内后果和PECAM-1 ITIM的功能；血栓调节蛋白在细胞生理学中的功能；幼年发作性血栓的小鼠模型；利用基因组技术促进鉴定淋巴细胞减少症/IDDM基因的生理作用；纤维蛋白抗凝血酶I活性；花瓶闭塞在镰刀细胞疾病中的机制；小鼠组织相容性抗原H60、H4和H71的人类同源基因的鉴定和鉴定；KU70在细胞凋亡调节中的新功能；Plcy1在B细胞发育和功能中的作用。购买毛细管DNA测序仪不仅将支持上述研究项目，还将扩大年轻研究人员、美国国立卫生研究院培训助学金支持的博士后研究员和在一带一路培训的研究生的能力。

DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of The Blood Center of Southeastern Wisconsin has an established, long-term commitment to blood-related research. More than 15 Principle Investigators lead research programs in the fields of cardiovascular biology, immunology, immunohematology, immunoregulation, control of bleeding and thrombosis and transfusion medicine. Six Core Laboratories (Molecular Biology, Protein Chemistry, Hybridoma, Flow Cytometry, Biophysics, and Histology) provide research services for the laboratories at the BRI. Experienced technologists are responsible for the day-to-day operation of the Core Labs and provide the services requested by investigators. Each Core Lab has a Scientific Advisor who is a BRI Investigator with experience in the technology and services provided. The Manager of Core Labs oversees the operation of these support labs. DNA Sequencing is a service provided by the Molecular Biology Core Lab. To meet the needs of BRI researchers we are requesting funds to purchase an ABI Prism 3100 Genetic Analyzer. Projects initiated by nine NIH-funded investigators and those initiated by three new investigators require this facility to accomplish their research goals. These project titles are: Identification of Rare Platelet-Specific AIIoantigens that Trigger Neonatal AIIoimmune Thrombocytopenia; Characterization of Recombinant Fab Fragments that Mimic the Behavior of Drug-Dependent Antibodies; Molecular Basis of A and B Blood Group Antigen Expression of Platelets; Structure and Function of vWAgll; Molecular and Cellular Consequences of vWf Alteration; Role of GPIb in Thrombotic Mechanisms; Integrative Approach to Coronary Thrombosis; Protein Sorting to Endothelial Cells Secretory Granules; Analysis of T cell Responses to Platelet AIIoantigens; Function of HLA-DR Polymorphisms Studied by Mutagenesis; Modulating GVH/GVL Post BMT Using TK Expressing T Cells; Characterization of the Molecular Requirements for Regulation of Cellular Adhesion by PECAM-1 ; Establishment of Mouse Models to Study the In Vivo Consequences of Constitutive Integrin Activation and Functions of the PECAM-1 ITIM; Thrombomodulin Function in Cellular Physiology; Mouse Model of Juvenile Onset Thrombosis; Use of Genomic Technologies to Facilitate the Identification of the Lymphopenia/IDDM Gene Physiological Role of Fibrin Anti-Thrombin I Activities; Mechanisms of Vase-Occlusion in Sickle Cell Disease; Identification and Characterization of Human Homologs of Murine Histocompatibility Antigens, H60, H4, and H71; New Function of KU70 in Apoptosis Regulation; Role of PLCy1 in B Cell Development and Function. The acquisition of a capillary DNA sequencer will not only support the research projects described above but will also broaden the capabilities of younger investigators, post-doctoral fellows supported by our NIH Training Grant and graduate students who train at the BRI.