Capillary DNA Sequencer/Genetic Analyzer

毛细管 DNA 测序仪/基因分析仪

基本信息

  • 批准号:
    6578397
  • 负责人:
  • 金额:
    $ 14.2万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2003
  • 资助国家:
    美国
  • 起止时间:
    2003-04-01 至 2004-03-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of The Blood Center of Southeastern Wisconsin has an established, long-term commitment to blood-related research. More than 15 Principle Investigators lead research programs in the fields of cardiovascular biology, immunology, immunohematology, immunoregulation, control of bleeding and thrombosis and transfusion medicine. Six Core Laboratories (Molecular Biology, Protein Chemistry, Hybridoma, Flow Cytometry, Biophysics, and Histology) provide research services for the laboratories at the BRI. Experienced technologists are responsible for the day-to-day operation of the Core Labs and provide the services requested by investigators. Each Core Lab has a Scientific Advisor who is a BRI Investigator with experience in the technology and services provided. The Manager of Core Labs oversees the operation of these support labs. DNA Sequencing is a service provided by the Molecular Biology Core Lab. To meet the needs of BRI researchers we are requesting funds to purchase an ABI Prism 3100 Genetic Analyzer. Projects initiated by nine NIH-funded investigators and those initiated by three new investigators require this facility to accomplish their research goals. These project titles are: Identification of Rare Platelet-Specific AIIoantigens that Trigger Neonatal AIIoimmune Thrombocytopenia; Characterization of Recombinant Fab Fragments that Mimic the Behavior of Drug-Dependent Antibodies; Molecular Basis of A and B Blood Group Antigen Expression of Platelets; Structure and Function of vWAgll; Molecular and Cellular Consequences of vWf Alteration; Role of GPIb in Thrombotic Mechanisms; Integrative Approach to Coronary Thrombosis; Protein Sorting to Endothelial Cells Secretory Granules; Analysis of T cell Responses to Platelet AIIoantigens; Function of HLA-DR Polymorphisms Studied by Mutagenesis; Modulating GVH/GVL Post BMT Using TK Expressing T Cells; Characterization of the Molecular Requirements for Regulation of Cellular Adhesion by PECAM-1 ; Establishment of Mouse Models to Study the In Vivo Consequences of Constitutive Integrin Activation and Functions of the PECAM-1 ITIM; Thrombomodulin Function in Cellular Physiology; Mouse Model of Juvenile Onset Thrombosis; Use of Genomic Technologies to Facilitate the Identification of the Lymphopenia/IDDM Gene Physiological Role of Fibrin Anti-Thrombin I Activities; Mechanisms of Vase-Occlusion in Sickle Cell Disease; Identification and Characterization of Human Homologs of Murine Histocompatibility Antigens, H60, H4, and H71; New Function of KU70 in Apoptosis Regulation; Role of PLCy1 in B Cell Development and Function. The acquisition of a capillary DNA sequencer will not only support the research projects described above but will also broaden the capabilities of younger investigators, post-doctoral fellows supported by our NIH Training Grant and graduate students who train at the BRI.
描述(由申请人提供):威斯康星州东南部血液中心的血液研究所(BRI)长期致力于血液相关研究。超过15名主要研究员在心血管生物学、免疫学、免疫血液学、免疫调节、出血和血栓控制以及输血医学等领域领导研究项目。六个核心实验室(分子生物学、蛋白质化学、杂交瘤、流式细胞术、生物物理学和组织学)为BRI的实验室提供研究服务。经验丰富的技术人员负责核心实验室的日常运作,并提供调查人员要求的服务。每个核心实验室都有一名科学顾问,他是一名具有所提供技术和服务经验的BRI调查员。核心实验室经理负责监督这些支持实验室的运作。DNA测序是分子生物学核心实验室提供的一项服务。为了满足BRI研究人员的需求,我们正在申请资金购买ABI Prism 3100基因分析仪。由9名nih资助的研究人员发起的项目和由3名新研究人员发起的项目需要这个设施来完成他们的研究目标。这些项目的标题是:鉴定引发新生儿血小板免疫性血小板减少症的罕见血小板特异性抗原;模拟药物依赖性抗体行为的重组Fab片段的表征血小板A、B血型抗原表达的分子基础vWAgll的结构与功能vWf改变对分子和细胞的影响GPIb在血栓形成机制中的作用冠状动脉血栓形成的综合治疗内皮细胞分泌颗粒的蛋白质分选T细胞对血小板AIIoantigens的反应分析HLA-DR多态性的诱变功能研究TK表达的T细胞调节BMT后GVH/GVLPECAM-1调控细胞粘附的分子需求表征构建小鼠模型研究组成性整合素激活的体内效应及PECAM-1 ITIM的功能凝血调节蛋白在细胞生理学中的作用小鼠幼年性血栓形成模型的建立利用基因组技术鉴定纤维蛋白抗凝血酶I活性对淋巴细胞减少/IDDM基因的生理作用镰状细胞病花瓶闭塞机制的研究鼠组织相容性抗原H60、H4和H71人同源物的鉴定与鉴定KU70在细胞凋亡调控中的新功能PLCy1在B细胞发育和功能中的作用。获得毛细管DNA测序仪不仅将支持上述研究项目,还将扩大年轻研究者、NIH培训基金支持的博士后研究员和在“一带一路”培训的研究生的能力。

项目成果

期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
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Lester William Cashdollar其他文献

Lester William Cashdollar的其他文献

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{{ truncateString('Lester William Cashdollar', 18)}}的其他基金

Spinning Disk Confocal Microscope System
转盘共焦显微镜系统
  • 批准号:
    8640561
  • 财政年份:
    2014
  • 资助金额:
    $ 14.2万
  • 项目类别:
RESEARCH IRRADIATOR: IMMUNOLOGY
研究辐照器:免疫学
  • 批准号:
    7334997
  • 财政年份:
    2006
  • 资助金额:
    $ 14.2万
  • 项目类别:
RESEARCH IRRADIATOR: INFLUENZA
研究辐射器:流感
  • 批准号:
    7334996
  • 财政年份:
    2006
  • 资助金额:
    $ 14.2万
  • 项目类别:
RESEARCH IRRADIATOR: MULTIPLE SCLEROSIS
研究照射器:多发性硬化症
  • 批准号:
    7334994
  • 财政年份:
    2006
  • 资助金额:
    $ 14.2万
  • 项目类别:
Research Irradiator
研究辐照器
  • 批准号:
    7046427
  • 财政年份:
    2006
  • 资助金额:
    $ 14.2万
  • 项目类别:
RESEARCH IRRADIATOR: SICKLE CELL, CANCER
研究辐照器:镰状细胞、癌症
  • 批准号:
    7334995
  • 财政年份:
    2006
  • 资助金额:
    $ 14.2万
  • 项目类别:
Shared Instrumentation Core
共享仪器核心
  • 批准号:
    7140697
  • 财政年份:
    2005
  • 资助金额:
    $ 14.2万
  • 项目类别:
Viral Vector Core
病毒载体核心
  • 批准号:
    6847311
  • 财政年份:
    2004
  • 资助金额:
    $ 14.2万
  • 项目类别:
HLA-Transgenic Mouse Core
HLA-转基因小鼠核心
  • 批准号:
    6847309
  • 财政年份:
    2004
  • 资助金额:
    $ 14.2万
  • 项目类别:
HLA-Transgenic Mouse Core
HLA-转基因小鼠核心
  • 批准号:
    7494621
  • 财政年份:
  • 资助金额:
    $ 14.2万
  • 项目类别:
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