Capillary DNA Sequencer/Genetic Analyzer

描述（由申请人提供）：威斯康星州东南部血液中心的血液研究所（BRI）对血液相关研究有着长期的承诺。超过15名首席研究员领导心血管生物学，免疫学，免疫血液学，免疫调节，出血和血栓形成控制以及输血医学领域的研究项目。六个核心实验室（分子生物学，蛋白质化学，杂交，流式细胞术，生物物理学和组织学）为BRI的实验室提供研究服务。经验丰富的技术专家负责核心实验室的日常运作，并提供研究者要求的服务。每个核心实验室都有一名科学顾问，他是BRI研究员，在所提供的技术和服务方面具有经验。核心实验室经理负责监督这些支持实验室的运行。DNA测序是由分子生物学核心实验室提供的服务。为了满足BRI研究人员的需求，我们正在申请资金购买ABI Prism 3100基因分析仪。由9名NIH资助的研究人员发起的项目和由3名新研究人员发起的项目需要该设施来实现其研究目标。这些项目的名称是：引发新生儿全免疫性血小板减少症的罕见血小板特异性全抗原的鉴定;模拟药物依赖性抗体行为的重组Fab B片段的表征;血小板A和B血型抗原表达的分子基础; vWAgll的结构和功能; vWf改变的分子和细胞后果; GPI B在血栓形成机制中的作用;冠状动脉血栓形成的综合治疗方法;内皮细胞分泌颗粒的蛋白分选; T细胞对血小板全抗原反应的分析;通过突变研究HLA-DR多态性的功能;使用表达TK的T细胞调节BMT后的GVH/GVL; PECAM-1调节细胞粘附的分子要求的表征;研究PECAM-1 ITIM的组成性整合素激活和功能的体内结果的小鼠模型的建立;血栓调节蛋白在细胞生理学中的功能;幼年发作血栓形成的小鼠模型;利用基因组技术鉴定淋巴细胞减少症/胰岛素依赖型糖尿病基因;镰状细胞病血管闭塞的机制;小鼠组织相容性抗原H60、H4和H71的人类同源物的鉴定和表征; KU 70在凋亡调节中的新功能; PLC γ 1在B细胞发育和功能中的作用。毛细管DNA测序仪的收购不仅将支持上述研究项目，而且还将扩大年轻研究人员的能力，由我们的NIH培训补助金支持的博士后研究员和在BRI培训的研究生。

DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of The Blood Center of Southeastern Wisconsin has an established, long-term commitment to blood-related research. More than 15 Principle Investigators lead research programs in the fields of cardiovascular biology, immunology, immunohematology, immunoregulation, control of bleeding and thrombosis and transfusion medicine. Six Core Laboratories (Molecular Biology, Protein Chemistry, Hybridoma, Flow Cytometry, Biophysics, and Histology) provide research services for the laboratories at the BRI. Experienced technologists are responsible for the day-to-day operation of the Core Labs and provide the services requested by investigators. Each Core Lab has a Scientific Advisor who is a BRI Investigator with experience in the technology and services provided. The Manager of Core Labs oversees the operation of these support labs. DNA Sequencing is a service provided by the Molecular Biology Core Lab. To meet the needs of BRI researchers we are requesting funds to purchase an ABI Prism 3100 Genetic Analyzer. Projects initiated by nine NIH-funded investigators and those initiated by three new investigators require this facility to accomplish their research goals. These project titles are: Identification of Rare Platelet-Specific AIIoantigens that Trigger Neonatal AIIoimmune Thrombocytopenia; Characterization of Recombinant Fab Fragments that Mimic the Behavior of Drug-Dependent Antibodies; Molecular Basis of A and B Blood Group Antigen Expression of Platelets; Structure and Function of vWAgll; Molecular and Cellular Consequences of vWf Alteration; Role of GPIb in Thrombotic Mechanisms; Integrative Approach to Coronary Thrombosis; Protein Sorting to Endothelial Cells Secretory Granules; Analysis of T cell Responses to Platelet AIIoantigens; Function of HLA-DR Polymorphisms Studied by Mutagenesis; Modulating GVH/GVL Post BMT Using TK Expressing T Cells; Characterization of the Molecular Requirements for Regulation of Cellular Adhesion by PECAM-1 ; Establishment of Mouse Models to Study the In Vivo Consequences of Constitutive Integrin Activation and Functions of the PECAM-1 ITIM; Thrombomodulin Function in Cellular Physiology; Mouse Model of Juvenile Onset Thrombosis; Use of Genomic Technologies to Facilitate the Identification of the Lymphopenia/IDDM Gene Physiological Role of Fibrin Anti-Thrombin I Activities; Mechanisms of Vase-Occlusion in Sickle Cell Disease; Identification and Characterization of Human Homologs of Murine Histocompatibility Antigens, H60, H4, and H71; New Function of KU70 in Apoptosis Regulation; Role of PLCy1 in B Cell Development and Function. The acquisition of a capillary DNA sequencer will not only support the research projects described above but will also broaden the capabilities of younger investigators, post-doctoral fellows supported by our NIH Training Grant and graduate students who train at the BRI.