RESEARCH IRRADIATOR: IMMUNOLOGY

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：威斯康星州东南部血液中心的血液研究所（BRI）长期致力于血液相关研究。超过15名主要研究员在心血管生物学、免疫学、癌症生物学、出血和血栓控制以及输血医学等领域领导研究项目。许多这样的研究项目在各种人类疾病模型中使用转基因、基因敲除和正常小鼠。用这些动物进行的实验通常需要使用伽玛辐照器进行部分或致死照射。由于BRI科学家越来越多地使用小鼠，而密尔沃基地区医学院缺乏研究动物空间，血液中心已与威斯康星医学院达成协议，将BRI科学家所需的小鼠安置在新的MCW生物医学资源中心。在这个新的动物设施中，将需要一个伽马辐照器作为共用设备。我们在此申请中请求资金用于购买研究用伽玛辐照器。由14位nih资助的研究者发起的项目和由一位新研究者发起的项目需要该仪器来完成他们的研究目标。项目名称为：VWFpp的结构与功能；vWF改变对分子和细胞的影响人类对流感的强大T细胞免疫血小板活化与粘附的分子机制研究PECAM-1的分子生物学及功能研究PECAM-1介导的内皮氧化还原信号传导镰状细胞病血管闭塞机制：血栓形成的作用镰状细胞病红细胞黏附的研究PLCyl和PLCy2在T细胞活化和发育中的单独和协同作用Shp-2对StatS的去磷酸化；骨髓来源的Allo-NK细胞用于肿瘤靶向免疫治疗隐表位在CD8+ T细胞介导的肿瘤清除中的作用重要MH-Ag， H60在GvL中的新功能致脑性T细胞对小胶质细胞的调控作用BCR/ABL研究Bcl-x在白血病发生中的作用内皮组织因子途径抑制因子结合蛋白的研究CD200在凋亡dc中的表达与免疫耐受CD4调节性T细胞抑制骨髓移植后的同种异体反应神经母细胞瘤脑转移后免疫治疗的优化γ δ T细胞对移植和免疫的调控输血医学中的分子和细胞机制，核心C，转基因核心。伽马辐照器是MCW新动物设施所需的重要共享设备，BRI和MCW调查员的小鼠将被安置在这里。它将支持现有研究人员的需求，并将在血液研究所和威斯康星医学院计划扩张期间招募新的研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of The Blood Center of Southeastern Wisconsin has an established, long-term commitment to blood-related research. More than 15 Principle Investigators lead research programs in the fields of cardiovascular biology, immunology, cancer biology, control of bleeding and thrombosis and transfusion medicine. Many of these research programs utilize transgenic, knockout and normal mice in various models of human diseases. Experiments performed with these animals often require partial or lethal irradiation using a gamma irradiator. Because of the increased use of mice by BRI scientists and the lack of research animal space on the Milwaukee Regional Medical campus the Blood Center has entered into an agreement with the Medical College of Wisconsin to house the mice needed by BRI scientists in the new MCW Biomedical Resource Center. A gamma irradiator will be needed as shared equipment in this new animal facility. We request funds in this application to purchase a research gamma irradiator. Projects initiated by fourteen NIH-funded investigators and those initiated by one new investigator require this instrument to accomplish their research goals. These project titles are: Structure and Function of VWFpp; Molecular and Cellular Consequences of vWF Alteration; Robust T cell Immunity to Influenza in Human Populations; Molecular Mechanisms of Platelet Activation and Adhesion; Molecular Biology and Function of PECAM-1; Endothelial Redox Signaling Mediated by PECAM-1; Mechanisms of Vaso-Occlusion in Sickle Cell Disease: role of thrombosis; Red Blood Cell Adhesion in Sickle Cell Disease; Individual and Collaborative Roles of PLCyl and PLCy2 in T cell Activation and Development; StatS Dephosphorylation by Shp-2; Bone Marrow Derived Allo-NK Cells for Tumor Targeted Immunotherapy; The Role of Cryptic Epitopes in CD8+ T cell Mediated Tumor Clearance; Novel Function of an Important MH-Ag, H60 during GvL; Encephalitogenic T Cell Regulation of Microglial Cells; Investigating the Role of Bcl-x in Leukemogenesis by BCR/ABL; Tissue Factor Pathway Inhibitor Binding Proteins on Endothelium; CD200 Expression on Apoptotic DCs and Immune Tolerance; CD4 Regulatory T Cells Suppress Alloreactivity after BMT; Optimizing Immunotherapy for Neuroblastoma after BMT; Regulation of Engraftment and Immunity by Gamma Delta T Cells; Molecular and Cellular Mechanisms in Transfusion Medicine, Core C, Transgenic Core. A gamma irradiator is an essential piece of shared equipment that is needed for the new MCW animal facility where BRI and MCW Investigator's mice will be housed. It will support the needs of current investigators and will be available to new investigators as they are recruited during the planned expansion of the Blood Research Institute and the Medical of Wisconsin.