Spinning Disk Confocal Microscope System

转盘共焦显微镜系统

• 批准号: 8640561
• 负责人: Lester William Cashdollar
• 金额: $ 43.17万
• 依托单位: VERSITI WISCONSIN, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640561
• 关键词: Adhesions; Affect; Angiogenesis Inhibitors; Animal Model; Asthma; B-Lymphocytes; Biochemistry; Biology; Blood; Blood Platelets; Blood Vessels; Blood flow; CD31 Antigens; CD36 gene; Cardiovascular system; Cellular biology; Data; Diagnosis; Disease; Endothelium; Funding; Goals; Hematological Disease; Hemophilia A; Hemorrhage; Heparin; Immunology; In Vitro; Injury; Integrins; Lead; Life; Medicine; Microscope; Minor; Molecular; Molecular Biology; Myocardial Infarction; Nociception; Pain; Physiological; Physiology; Platelet Activation; Principal Investigator; Radiation; Regulation; Research; Research Institute; Research Personnel; Role; Scientist; Sepsis; Sickle Cell; Sickle Cell Anemia; Signal Transduction; Stroke; System; TFPI; Thrombocytopenia; Thrombophilia; Thrombosis; Thrombus; Transfusion; United States National Institutes of Health; Vascular Diseases; Wisconsin; activated Protein C; comparative effectiveness; gene therapy; human disease; in vivo; instrument; intravital imaging; programs; research study; response; stem cell biology; tool

DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of BloodCenter of Wisconsin has an established, long-term commitment to blood-related research. Currently 19 Principal Investigators lead research programs in the fields of cardiovascular biology, immunology, stem cell biology, transfusion medicine, control of bleeding and thrombosis. The BRI has had a tradition of research strength in platelet and vascular cell biology and have applied their research findings to the treatment of bleeding disorders, blood diseases, heart attack and stroke. The research programs of many BRI investigators have moved into studies involving various animal models of human disease. Experiments performed in vivo require specialized tools to obtain data under physiological relevant conditions. We request funds to purchase a spinning disk confocal microscope system for intravital imaging to accomplish the research goals described by the major and minor users in this application. Projects initiated by twelve NIH-funded Investigators and those initiated by two new investigators require this instrument to accomplish their research goals. These project titles are: Molecular biology and function of PECAM-1; Molecular mechanisms of platelet activation and adhesion; Biomolecular interactions of FVIII and VWF; Molecular impact of VWF on VWD; Comparative effectiveness in the diagnosis of VWD; Asthma increases vaso-occlusion in sickle cell disease; Nociceptive mechanism underlying sickle cell pain; Negative regulation of platelet activity; Molecular mechanisms of VWF alteration in vitro/vivo; B-cell responses in heparin induced thrombocytopenia; Regulation of the anti- antiangiogenic switch by CD36 in thrombosis; Mechanistic role of CD36 signaling in thrombosis; Association of TFPI with endothelium; The biochemistry and physiology of platelet TFPI; Activated Protein C for Treatment of Radiation Combined Injury; Mechanism of Activated Protein C Action in Sepsis Therapy; Platelet- derived FVIII gene therapy of hemophilia A; Mechanism of kindlin-2 in B3 integrin activation; Platelet Function in Thrombophilia-Associated Placental Vascular Disorder. The spinning disk confocal microscope system will become a part of the newly formed Thrombosis Core Lab at the BRI and will enable scientists to evaluate various experimental parameters that affect blood flow and thrombus formation in live animal models.

描述（由申请人提供）：威斯康星州血液中心的血液研究所（BRI）对血液相关研究有着长期的承诺。目前，19名主要研究者领导心血管生物学、免疫学、干细胞生物学、输血医学、出血和血栓控制等领域的研究项目。BRI在血小板和血管细胞生物学方面具有研究实力的传统，并将其研究成果应用于出血性疾病，血液病，心脏病发作和中风的治疗。许多BRI研究人员的研究项目已经进入了涉及各种人类疾病动物模型的研究。在体内进行的实验需要专门的工具来获得生理相关条件下的数据。我们要求资金购买一个旋转盘共聚焦显微镜系统的活体成像，以完成本申请中的主要和次要用户所描述的研究目标。由12名NIH资助的研究人员发起的项目和由两名新研究人员发起的项目需要该仪器来实现其研究目标。这些项目的名称是：PECAM-1的分子生物学和功能;血小板活化和粘附的分子机制; FVIII和VWF的生物分子相互作用; VWF对VWD的分子影响; VWD诊断的比较有效性;哮喘增加镰状细胞病的血管闭塞;镰状细胞疼痛的伤害性机制;血小板活性的负调节;体外/体内VWF改变的分子机制;肝素诱导的血小板减少症中的B细胞反应;血栓形成中CD 36对抗抗血管生成开关的调节;血栓形成中CD 36信号传导的机制作用; TFPI与内皮的关系;血小板TFPI的生物化学和生理学;放射复合损伤治疗中的活化蛋白C;脓毒症治疗中活化蛋白C的作用机制;血友病A的血小板源性FVIII基因治疗; Kindlin-2在B3整合素活化中的机制;血小板功能在血栓形成相关胎盘血管疾病中的作用。旋转圆盘共聚焦显微镜系统将成为BRI新成立的血栓形成核心实验室的一部分，使科学家能够评估影响活体动物模型中血流和血栓形成的各种实验参数。