Spinning Disk Confocal Microscope System

描述（由申请人提供）：威斯康星州血液中心的血液研究所（BRI）长期致力于血液相关研究。目前有19名首席研究员在心血管生物学、免疫学、干细胞生物学、输血医学、出血和血栓控制等领域领导研究项目。“一带一路”在血小板和血管细胞生物学方面具有较强的研究实力，并将研究成果应用于出血性疾病、血液病、心脏病和中风的治疗。许多BRI研究人员的研究项目已经转移到涉及各种人类疾病动物模型的研究中。在体内进行的实验需要专门的工具来获得生理相关条件下的数据。我们申请资金购买旋转盘共聚焦显微镜系统用于活体成像，以完成本应用程序中主要和次要用户描述的研究目标。由12位美国国立卫生研究院资助的研究人员发起的项目和由两位新研究人员发起的项目需要该仪器来完成他们的研究目标。项目名称为：PECAM-1的分子生物学和功能；血小板活化与粘附的分子机制FVIII与VWF的生物分子相互作用VWF对VWD的分子影响VWD诊断的比较疗效分析哮喘增加镰状细胞病患者的血管闭塞；镰状细胞痛的伤害感受机制；血小板活性负调控；体外/体内VWF改变的分子机制肝素诱导的血小板减少症中的b细胞反应CD36在血栓形成中对抗血管生成开关的调控CD36信号在血栓形成中的作用机制TFPI与内皮细胞的关系血小板TFPI的生理生化研究活化蛋白C在放射复合损伤治疗中的应用活化蛋白C在脓毒症治疗中的作用机制血小板源性FVIII基因治疗A型血友病kindlin-2在B3整合素活化中的作用机制血小板功能与血栓相关的胎盘血管紊乱。旋转盘共聚焦显微镜系统将成为BRI新成立的血栓形成核心实验室的一部分，使科学家能够在活体动物模型中评估影响血流和血栓形成的各种实验参数。

DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of BloodCenter of Wisconsin has an established, long-term commitment to blood-related research. Currently 19 Principal Investigators lead research programs in the fields of cardiovascular biology, immunology, stem cell biology, transfusion medicine, control of bleeding and thrombosis. The BRI has had a tradition of research strength in platelet and vascular cell biology and have applied their research findings to the treatment of bleeding disorders, blood diseases, heart attack and stroke. The research programs of many BRI investigators have moved into studies involving various animal models of human disease. Experiments performed in vivo require specialized tools to obtain data under physiological relevant conditions. We request funds to purchase a spinning disk confocal microscope system for intravital imaging to accomplish the research goals described by the major and minor users in this application. Projects initiated by twelve NIH-funded Investigators and those initiated by two new investigators require this instrument to accomplish their research goals. These project titles are: Molecular biology and function of PECAM-1; Molecular mechanisms of platelet activation and adhesion; Biomolecular interactions of FVIII and VWF; Molecular impact of VWF on VWD; Comparative effectiveness in the diagnosis of VWD; Asthma increases vaso-occlusion in sickle cell disease; Nociceptive mechanism underlying sickle cell pain; Negative regulation of platelet activity; Molecular mechanisms of VWF alteration in vitro/vivo; B-cell responses in heparin induced thrombocytopenia; Regulation of the anti- antiangiogenic switch by CD36 in thrombosis; Mechanistic role of CD36 signaling in thrombosis; Association of TFPI with endothelium; The biochemistry and physiology of platelet TFPI; Activated Protein C for Treatment of Radiation Combined Injury; Mechanism of Activated Protein C Action in Sepsis Therapy; Platelet- derived FVIII gene therapy of hemophilia A; Mechanism of kindlin-2 in B3 integrin activation; Platelet Function in Thrombophilia-Associated Placental Vascular Disorder. The spinning disk confocal microscope system will become a part of the newly formed Thrombosis Core Lab at the BRI and will enable scientists to evaluate various experimental parameters that affect blood flow and thrombus formation in live animal models.