RESEARCH IRRADIATOR: SICKLE CELL, CANCER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请者提供)：威斯康星州东南部血液中心血液研究所(BRI)对血液相关研究有既定的、长期的承诺。超过15名主要研究人员领导心血管生物学、免疫学、癌症生物学、出血和血栓控制以及输血医学等领域的研究项目。其中许多研究项目利用转基因、基因敲除和正常小鼠在各种人类疾病模型中进行研究。在这些动物身上进行的实验通常需要使用伽马辐照器进行部分或致命的照射。由于密尔沃基地区医学院老鼠使用量的增加和研究动物空间的缺乏，血液中心已与威斯康星州医学院达成协议，将在新的MCW生物医学资源中心安置一带一路科学家所需的小鼠。在这个新的动物设施中，将需要一个伽马辐射器作为共享设备。我们在这个应用程序中请求资金来购买一个研究型伽马辐射器。由NIH资助的14名研究人员发起的项目和由一名新研究人员发起的项目需要这一工具来实现他们的研究目标。这些项目的题目是：VWFpp的结构和功能；vWF改变的分子和细胞后果；人类对流感的强大T细胞免疫；血小板激活和黏附的分子机制；PECAM-1的分子生物学和功能；PECAM-1介导的内皮氧化还原信号；血管闭塞在镰状细胞疾病中的机制：血栓形成的作用；红细胞在镰状细胞疾病中的黏附；PLCyl和PLCy2在T细胞激活和发育中的单独和协同作用；SHP-2的STATS去磷酸化；骨髓来源的异基因NK细胞用于肿瘤靶向免疫治疗；隐蔽表位在CD8+T细胞介导的肿瘤清除中的作用；重要的MH-Ag，H60在GVL中的新功能；生脑T细胞对小胶质细胞的调节；BCR/ABL研究Bcl-x在白血病发生中的作用；内皮上组织因子途径抑制物结合蛋白；CD200在凋亡的DC上的表达与免疫耐受；CD4调节T细胞抑制骨髓移植后的同种异体反应；优化BMT后神经母细胞瘤的免疫治疗；Gamma Delta T细胞对植入和免疫的调节；输血医学，核心C，转基因核心中的分子和细胞机制。伽马辐射器是新的MCW动物设施所需的基本共享设备，BRI和MCW Investigator的小鼠将在这里居住。它将支持现有调查人员的需求，并将提供给新的调查人员，因为他们是在计划扩大血液研究所和威斯康星州医学研究所期间招聘的。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of The Blood Center of Southeastern Wisconsin has an established, long-term commitment to blood-related research. More than 15 Principle Investigators lead research programs in the fields of cardiovascular biology, immunology, cancer biology, control of bleeding and thrombosis and transfusion medicine. Many of these research programs utilize transgenic, knockout and normal mice in various models of human diseases. Experiments performed with these animals often require partial or lethal irradiation using a gamma irradiator. Because of the increased use of mice by BRI scientists and the lack of research animal space on the Milwaukee Regional Medical campus the Blood Center has entered into an agreement with the Medical College of Wisconsin to house the mice needed by BRI scientists in the new MCW Biomedical Resource Center. A gamma irradiator will be needed as shared equipment in this new animal facility. We request funds in this application to purchase a research gamma irradiator. Projects initiated by fourteen NIH-funded investigators and those initiated by one new investigator require this instrument to accomplish their research goals. These project titles are: Structure and Function of VWFpp; Molecular and Cellular Consequences of vWF Alteration; Robust T cell Immunity to Influenza in Human Populations; Molecular Mechanisms of Platelet Activation and Adhesion; Molecular Biology and Function of PECAM-1; Endothelial Redox Signaling Mediated by PECAM-1; Mechanisms of Vaso-Occlusion in Sickle Cell Disease: role of thrombosis; Red Blood Cell Adhesion in Sickle Cell Disease; Individual and Collaborative Roles of PLCyl and PLCy2 in T cell Activation and Development; StatS Dephosphorylation by Shp-2; Bone Marrow Derived Allo-NK Cells for Tumor Targeted Immunotherapy; The Role of Cryptic Epitopes in CD8+ T cell Mediated Tumor Clearance; Novel Function of an Important MH-Ag, H60 during GvL; Encephalitogenic T Cell Regulation of Microglial Cells; Investigating the Role of Bcl-x in Leukemogenesis by BCR/ABL; Tissue Factor Pathway Inhibitor Binding Proteins on Endothelium; CD200 Expression on Apoptotic DCs and Immune Tolerance; CD4 Regulatory T Cells Suppress Alloreactivity after BMT; Optimizing Immunotherapy for Neuroblastoma after BMT; Regulation of Engraftment and Immunity by Gamma Delta T Cells; Molecular and Cellular Mechanisms in Transfusion Medicine, Core C, Transgenic Core. A gamma irradiator is an essential piece of shared equipment that is needed for the new MCW animal facility where BRI and MCW Investigator's mice will be housed. It will support the needs of current investigators and will be available to new investigators as they are recruited during the planned expansion of the Blood Research Institute and the Medical of Wisconsin.