Research Irradiator

研究辐照器

• 批准号: 7046427
• 负责人: Lester William Cashdollar
• 金额: $ 32.38万
• 依托单位: VERSITI WISCONSIN, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2008-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7046427
• 关键词: blood; role

DESCRIPTION (provided by applicant): The Blood Research Institute (BRI) of The Blood Center of Southeastern Wisconsin has an established, long-term commitment to blood-related research. More than 15 Principle Investigators lead research programs in the fields of cardiovascular biology, immunology, cancer biology, control of bleeding and thrombosis and transfusion medicine. Many of these research programs utilize transgenic, knockout and normal mice in various models of human diseases. Experiments performed with these animals often require partial or lethal irradiation using a gamma irradiator. Because of the increased use of mice by BRI scientists and the lack of research animal space on the Milwaukee Regional Medical campus the Blood Center has entered into an agreement with the Medical College of Wisconsin to house the mice needed by BRI scientists in the new MCW Biomedical Resource Center. A gamma irradiator will be needed as shared equipment in this new animal facility. We request funds in this application to purchase a research gamma irradiator. Projects initiated by fourteen NIH-funded investigators and those initiated by one new investigator require this instrument to accomplish their research goals. These project titles are: Structure and Function of VWFpp; Molecular and Cellular Consequences of vWF Alteration; Robust T cell Immunity to Influenza in Human Populations; Molecular Mechanisms of Platelet Activation and Adhesion; Molecular Biology and Function of PECAM-1; Endothelial Redox Signaling Mediated by PECAM-1; Mechanisms of Vaso-Occlusion in Sickle Cell Disease: role of thrombosis; Red Blood Cell Adhesion in Sickle Cell Disease; Individual and Collaborative Roles of PLCyl and PLCy2 in T cell Activation and Development; StatS Dephosphorylation by Shp-2; Bone Marrow Derived Allo-NK Cells for Tumor Targeted Immunotherapy; The Role of Cryptic Epitopes in CD8+ T cell Mediated Tumor Clearance; Novel Function of an Important MH-Ag, H60 during GvL; Encephalitogenic T Cell Regulation of Microglial Cells; Investigating the Role of Bcl-x in Leukemogenesis by BCR/ABL; Tissue Factor Pathway Inhibitor Binding Proteins on Endothelium; CD200 Expression on Apoptotic DCs and Immune Tolerance; CD4 Regulatory T Cells Suppress Alloreactivity after BMT; Optimizing Immunotherapy for Neuroblastoma after BMT; Regulation of Engraftment and Immunity by Gamma Delta T Cells; Molecular and Cellular Mechanisms in Transfusion Medicine, Core C, Transgenic Core. A gamma irradiator is an essential piece of shared equipment that is needed for the new MCW animal facility where BRI and MCW Investigator's mice will be housed. It will support the needs of current investigators and will be available to new investigators as they are recruited during the planned expansion of the Blood Research Institute and the Medical of Wisconsin.

描述（由申请人提供）：威斯康星州东南部血液中心的血液研究所（BRI）对血液相关研究有着长期的承诺。超过15名主要研究人员领导心血管生物学，免疫学，癌症生物学，出血和血栓形成控制以及输血医学领域的研究项目。许多这些研究计划利用转基因，基因敲除和正常小鼠在人类疾病的各种模型。用这些动物进行的实验通常需要使用伽马辐照器进行部分或致死辐照。由于BRI科学家越来越多地使用小鼠，而密尔沃基地区医学园区缺乏研究动物空间，血液中心已与威斯康星州医学院达成协议，将BRI科学家所需的小鼠安置在新的MCW生物医学资源中心。在这个新的动物设施中，需要一个伽马辐照器作为共用设备。我们在此申请资金购买研究伽马辐照器。由14名NIH资助的研究人员发起的项目和由一名新研究人员发起的项目需要该仪器来实现其研究目标。这些项目的名称是：VWFpp的结构和功能; vWF改变的分子和细胞后果;人群中对流感的强大T细胞免疫;血小板活化和粘附的分子机制; PECAM-1的分子生物学和功能; PECAM-1介导的内皮氧化还原信号传导;镰状细胞病中血管闭塞的机制：血栓形成的作用;镰状细胞病中的红细胞粘附; PLC γ 1和PLC γ 2在T细胞活化和发育中的单独和协同作用; Shp-2引起的StatS去磷酸化;用于肿瘤靶向免疫治疗的骨髓来源的同种异体NK细胞;隐蔽表位在CD 8 + T细胞介导的肿瘤清除中的作用;重要的MH-Ag，H60在GvL期间的新功能;致脑性T细胞对小胶质细胞的调节; Bcl-x在BCR/ABL白血病发生中的作用;内皮细胞上的组织因子途径抑制物结合蛋白;凋亡DC上的CD 200表达与免疫耐受; CD 4调节性T细胞抑制BMT后的同种异体反应;神经母细胞瘤BMT后免疫治疗的优化; γ δ T细胞对移植和免疫的调节;输血医学中的分子和细胞机制，核心C，转基因核心。伽马辐照器是新的MCW动物设施所需的共享设备的重要组成部分，BRI和MCW研究者的小鼠将在该设施中饲养。它将支持当前研究者的需求，并将提供给新的研究者，因为他们是在血液研究所和威斯康星州的医疗计划扩大招募。