HLA-Transgenic Mouse Core

HLA-转基因小鼠核心

• 批准号: 6847309
• 负责人: Lester William Cashdollar
• 金额: $ 7.09万
• 依托单位: VERSITI WISCONSIN, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6847309
• 关键词: animal colony; cellular immunity; cooperative study; cytotoxic T lymphocyte; disease /disorder model; flow cytometry; genetically modified animals; genotype; helper T lymphocyte; immune response; immunologic memory; influenza; laboratory mouse; major histocompatibility complex; transfection /expression vector

The study of human immunology is often difficult because certain types of experiments can't be performed on humans for obvious ethical reasons. To facilitate human research, many scientists utilize animal models. The animal model of choice for immunologists is the mouse. This animal model is very useful because of the large number of reagents available as well as the creation of transgenic and knockout mice. Both types of mice are required for this program project grant. Transgenic carrying "humanized" MHC molecules will be iused to discover new epitopes for influenza proteins in project 2 and in project 3, they will be used to model human influenza infection in mice. Knockout mice are required so that the mouse endogenous MHC class I or II genes are not expressed in the MHC transgenic mice. Two projects will require some of the same strains of mice and procedures, so for continuity between the projects an animal core providing the necessary services is required. The specific aims of the animal core are: 1) to provide high quality housing, husbandry, and health care for the mice that are used in the studies proposed by Center investigators, 2) to provide a dedicated, stable space for the mice used in the proposed studies, and 3) to provide expertise in the delivery of viral vectors to mice to ensure proper biocontainment of the vectors and efficient immunization of experimental animals.

人类免疫学的研究通常是困难的，因为某些类型的实验由于明显的伦理原因不能在人类身上进行。为了促进人类研究，许多科学家利用动物模型。免疫学家选择的动物模型是小鼠。这种动物模型非常有用，因为有大量可用的试剂，以及创造了转基因和基因敲除小鼠。这两种类型的MICE都是本计划项目拨款所必需的。在项目2和项目3中，携带“人源化”MHC分子的转基因将被用来发现流感蛋白的新表位，它们将被用来模拟小鼠的人类流感感染。需要敲除小鼠才能使小鼠内源性MHC I类 或II基因在MHC转基因小鼠中不表达。两个项目将需要一些 同样的小鼠品系和程序，因此为了项目之间的连续性，需要一个提供必要服务的动物核心。动物核心的具体目标是：1)为中心研究人员建议的研究中使用的小鼠提供高质量的住房、饲养和医疗保健；2)为建议研究中使用的小鼠提供专门的、稳定的空间；3)提供向小鼠运送病毒载体的专业知识，以确保适当的生物遏制和对实验动物的有效免疫。