Development of Immunological Reagents for the Identification of New World Monkey Biomarkers
开发用于鉴定新世界猴生物标志物的免疫试剂
基本信息
- 批准号:10592259
- 负责人:
- 金额:$ 49.77万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-03-15 至 2026-01-31
- 项目状态:未结题
- 来源:
- 关键词:AgingAliquotAmino AcidsAnimal ModelAnimalsAntibodiesAotus primateAreaBehaviorBindingBiologicalBiological AssayBiological MarkersBiomedical ResearchBiotechnologyBreedingC-reactive proteinCXCL10 geneCXCR3 geneCallithrixCallithrix jacchus jacchusCebidaeCellsCercopithecidaeChronic DiseaseClinicalClinical TrialsCognitionCommunicable DiseasesDataDevelopmentEnzyme-Linked Immunosorbent AssayEpitope MappingEpitopesEukaryotic CellExposure toGastrointestinal HormonesGenerationsGenesGoalsGranzymeHaplorhiniHealthHomologous GeneHormonesHumanImmunologic TechniquesImmunologicsImmunology procedureInfectionInflammationInflammatoryInstitutionInsulinInterferon Type IIInterleukin-10Interleukin-4Interleukin-6InterventionLeptinLongevityLongitudinal StudiesMacacaMacaca mulattaMalariaMembrane GlycoproteinsMetabolicMetabolic hormoneMetabolic syndromeMetabolismModelingMonkeysMonoclonal AntibodiesMultiple SclerosisMusNational Institute of Allergy and Infectious DiseaseNational Institute of Mental HealthNeurodegenerative DisordersNeurologyNeurosciencesNight MonkeyObesityOutcomePathologyPlasmaPre-Clinical ModelPrimatesProcessProductionProteinsProtocols documentationR24ReagentRecombinant ProteinsRecombinantsRecommendationReportingReproductionResearchResearch PersonnelResourcesRodentSaimiriSamplingScienceScientistSocial BehaviorSouth AmericaSouth AmericanSpecificityStainsTechniquesTechnologyTestingTransfectionTransgenic OrganismsUnited StatesUnited States National Academy of SciencesUnited States National Institutes of HealthValidationVisionWestern Blottingadiponectinage related neurodegenerationassay developmentbiomarker identificationcross reactivitycytokineenzyme linked immunospot assaygenetic manipulationhuman diseasehuman modelimprovedinterestmurine monoclonal antibodynonhuman primatepreclinical studytoolvalidation studiesweb site
项目摘要
PROJECT SUMMARY
Marmosets, squirrel monkeys, and owl monkeys are New World Monkeys (NWM) studied extensively in
a broad array of research areas, including infection, vision, audition, social behavior, cognition,
neurodegenerative diseases, reproduction, and genetic manipulation. One critical shortcoming of the NWM
animal models is the limited number of immunological reagents that are currently available due to more than
33 million of years of evolutionary divergence. This scarcity of research tools reduces the translational value of
these nonhuman primates. The goal of this R24 application is to remedy this deficiency by generating
antibodies and validating immunological assays that identify important NWM biomarkers of inflammation and
metabolism, for which there is currently lack of reagents. Inflammation is a common process involved in aging,
obesity, and pathologies such as infectious diseases, multiple sclerosis, and age-related neurodegenerative
disorders, all of which are being modeled in these species. The NWM inflammatory biomarkers to be produced
include C-reactive protein (CRP), interleukin-4 (IL-4), IL-6, IL-10, interferon gamma-inducible protein 10
(CXCL10, IP-10), granzyme B, and the surface glycoprotein CD69. Gastrointestinal hormones to be produced,
critical for validation of studies of metabolic syndrome, include insulin, adiponectin, and leptin. In order to
accomplish our goal, we have assembled a team of scientists from academic and research institutions and a
biotechnology company specialized in the production of reagents of veterinary and human use. We will
produce NWM proteins in eukaryotic cells, and use the recombinant marmoset proteins to produce monoclonal
antibodies (mMabs) in mice (Specific Aim 1). We will then use the marmoset recombinant proteins to identify
epitope specificity of these mAbs, select the ones that also recognize squirrel monkey and owl monkey
homologues, and identify optimal antibody pairs for techniques such as ELISA, ELISPOT, Luminex, and
intracellular cytokine staining (ICS) assays (SA 2). Finally, these immunological assays will be validated with
samples obtained from plasma aliquots from NWM exposed to transient metabolic and inflammatory
challenges, and from ex vivo stimulated NWM cells (SA 3). At the end of this project, researchers working in
different NWM models of human disease will have access to research tools for improved validation of their
preclinical models. A resource website will be developed to disseminate the availability of reagents, to track the
impact of these reagents in biomedical studies, and to distribute protocols and SOPs. Development of these
reagents is of trans-NIH interest, will increase the translational value of marmoset, squirrel monkey and owl
monkey modles, and will expand the scientific data obtained from these animals.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Luis David Giavedoni其他文献
Luis David Giavedoni的其他文献
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{{ truncateString('Luis David Giavedoni', 18)}}的其他基金
Development of Immunological Reagents for the Identification of New World Monkey Biomarkers
开发用于鉴定新世界猴生物标志物的免疫试剂
- 批准号:
10807651 - 财政年份:2023
- 资助金额:
$ 49.77万 - 项目类别:
Development of Immunological Reagents for the Identification of New World Monkey Biomarkers
开发用于鉴定新世界猴生物标志物的免疫试剂
- 批准号:
10334632 - 财政年份:2022
- 资助金额:
$ 49.77万 - 项目类别:
LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES
用于非人类灵长类动物细胞因子定量的 LUMINEX 技术
- 批准号:
8357650 - 财政年份:2011
- 资助金额:
$ 49.77万 - 项目类别:
LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES
用于非人类灵长类动物细胞因子定量的 LUMINEX 技术
- 批准号:
8172652 - 财政年份:2010
- 资助金额:
$ 49.77万 - 项目类别:
LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES
用于非人类灵长类动物细胞因子定量的 LUMINEX 技术
- 批准号:
7957900 - 财政年份:2009
- 资助金额:
$ 49.77万 - 项目类别:
LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES
用于非人类灵长类动物细胞因子定量的 LUMINEX 技术
- 批准号:
7716084 - 财政年份:2008
- 资助金额:
$ 49.77万 - 项目类别:
IMMUNOGENICITY OF HSV AMPLICONS IN RHESUS MACAQUES
HSV 扩增子在恒河猴中的免疫原性
- 批准号:
7562431 - 财政年份:2007
- 资助金额:
$ 49.77万 - 项目类别:
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