BD FACSARIA FLOW CYTOMETER 3 LSR

描述（由申请人提供）：此申请是为购买FACSAria细胞分选机而申请的资金。该仪器将被安置在匹兹堡大学新成立的免疫学系。目前，没有细胞分选器可供该系的初级和许多二级教师使用，因此这次购买将对匹兹堡大学的免疫学社区产生巨大影响。匹兹堡大学现有的细胞分选机要么不提供给部门成员使用，要么位于离部门几英里远的地方。进行高速细胞分选的能力是许多免疫学研究的基本特征。因此，此申请的原因是：1)提供急需的仪器；2)为初级和次级免疫学教师提供一种易于使用的易于获取的细胞分选器；3)形成新创建的免疫学流动设施的基础。2002年1月，为了表彰匹兹堡大学免疫学研究的实力，一个新的免疫学系成立了。目前免疫学系有30名中小学教师，大部分由美国国立卫生研究院资助。获得所要求的单元分选机将使个别用户能够进行单元分选，目前该部的许多成员无法进行这种分选。

DESCRIPTION (provided by applicant): This request is for funds to purchase a FACSAria cell sorter. This instrument will be housed within the newly created Department of Immunology at the University of Pittsburgh. At present there is no cell sorter available for use by primary and many of the secondary faculty in the Department and thus this purchase will have an enormous impact on the Immunology community at the University of Pittsburgh. Existing cell sorters at the University of Pittsburgh are either not available to Department members or are located several miles away from the Department. The ability to perform high speed cell sorting is an essential feature of much immunology research. Thus the reasons for this application are to: 1) Provide a critically needed instrument; 2) provide an easy-to-use readily accessible cell sorter for primary and secondary Immunology faculty; and 3) form the basis of the newly created Immunology Flow Facility. In January 2002 a new Department of Immunology was created in recognition of the strength of the immunology research performed here at the University of Pittsburgh. At present there are 30 primary and secondary faculty in the Department of Immunology and most of them are NIH-funded. The acquisition of the requested cell sorter will allow individual users access to cell sorting, which is at present unavailable to many members of the Department.