Measurement of Cells In Vivo: IVIS Light Imaging System

描述(由申请人提供)： 此共享仪器赠款申请中要求使用Xenogen公司生产的IVIS成像系统。IVIS成像系统使用高效的CCD摄像机(在无光的成像室中)和Livingimage。快速捕获荧光素酶发出的光的软件，以成像人类或动物细胞、细菌、病毒或与荧光素酶表达相关的基因。该仪器将允许14名由NIH资助的研究人员使用人类疾病的动物模型完成并显著改进他们对肿瘤生长、传染病发病机制和体内基因表达的研究。重要的是，IVIS成像系统将使研究人员能够在不牺牲实验动物的情况下完成体内的纵向研究。该成像系统可以检测到皮下组织中多达200个细胞，并可以轻松地成像麻醉小鼠(一次最多10只)肺、淋巴结和骨骼中的细胞的荧光素酶表达。在小鼠身上进行的人类癌症的转译研究通常受到体内肿瘤测量或成像能力的限制。IVIS成像系统将能够准确测量皮下和内脏肿瘤中的活肿瘤细胞，评估治疗反应，并极大地缩短研究体内人类癌症的发病机制和治疗所需的时间和动物数量。此外，成像系统将允许研究人员监测体内的基因表达和病原体。这项技术将显著提高研究人员完成目前资助的研究项目的能力，并扩大研究人员提出新的转译研究项目的能力，这些项目需要在小鼠或其他小动物身上进行体内成像。俄亥俄州立大学没有类似的仪器，俄亥俄州只有一个IVIS成像系统(位于辛辛那提的宝洁公司)。该仪器将被安置在兽医学院的屏障动物设施(西森厅)，由一名研究科学家主管(专门从事成像)和一名实验室动物兽医监督。首席研究员在斯坦福大学博士克里斯·康塔格(IVIS系统的开发者和Xenogen公司的创始人)的实验室接受了IVIS成像系统的使用培训。使用者将接受首席调查员、辅助研究科学家和实验动物兽医的培训。该软件将在Windows环境下的联网计算机上运行，使调查人员能够从遥远的地点访问数据。每小时的使用费将为主管提供服务合同和继续教育的资金。计费将由兽医生物科学部成像服务的当前系统执行。兽医生物科学系和兽医学院将为该仪器提供场地和公用设施，以及主管的工资和福利。

DESCRIPTION (provided by applicant): An IVIS Imaging System made by Xenogen Corporation is requested in this Shared Instrument Grant application. The IVIS Imaging System uses a highly efficient CCD camera (in a light-free imaging chamber) and Livinglmage. Software to rapidly capture light emitted from luciferase to image human or animal cells, bacteria, viruses, or genes that are linked to luciferase expression. The instrument will allow fourteen NIH-funded investigators to complete and significantly improve their studies on tumor growth, pathogenesis of infectious disease, and gene expression in vivo using animal models of human disease. Importantly, the IVIS Imaging System will enable the investigators to complete longitudinal studies in vivo without sacrifice of experimental animals. The Imaging System can detect as few as 200 cells in the subcutis and can readily image luciferase expression by cells in lungs, lymph nodes, and bones of anesthetized mice (up to 10 mice at one time). Translational studies on human cancer in mice are usually limited by the ability to measure or image tumors in vivo. The IVIS Imaging System will permit accurate measurement of viable tumor cells in subcutaneous and visceral neoplasms, response to therapy, and greatly shorten the time and animals numbers needed to investigate the pathogenesis and treatment of human cancer in vivo. In addition, the Imaging System will allow investigators to monitor gene expression and pathogenic organisms in vivo. This technology will significantly improve the ability of the investigators to complete currently funded research projects and extend the capabilities of the investigators to propose new translational research projects that require in vivo imaging in mice or other small animals. There is no similar instrument at The Ohio State University, and only one IVIS Imaging System exists in Ohio (at Procter & Gamble Co., Cincinnati). The instrument will be housed in a barrier animal facility (Sisson Hall) in the College of Veterinary Medicine under the supervision of a Research Scientist Supervisor (that specializes in imaging) and a Laboratory Animal Veterinarian. The principal investigator has received training in use of the IVIS Imaging System in the laboratory of Chris Contag, PhD at Stanford University (developer of the IVIS System and founder of Xenogen Corp.). Users will be trained by the Principal Investigator and the supporting Research Scientist and Laboratory Animal Veterinarian. The software will operate in a Windows environment on a networked computer that will allow the investigators to access the data from distant sites. An hourly user fee will provide funds for a service contract and continuing education of the supervisor. Billing will be performed by the current system of the Imaging Services in the Department of Veterinary Biosciences. Space and utilities for the instrument plus salary and benefits for the supervisor will be provided by the Department of Veterinary Biosciences and the College of Veterinary Medicine.