Measurement of Cells In Vivo: IVIS Light Imaging System

描述（由申请人提供）： 在此共享仪器补助申请中，需要Xenogen Corporation制造的IVIS成像系统。IVIS成像系统使用高效CCD相机（在无光成像室中）和Livinglmage。快速捕获荧光素酶发出的光的软件，以成像人类或动物细胞，细菌，病毒或与荧光素酶表达相关的基因。该仪器将允许14名NIH资助的研究人员使用人类疾病的动物模型完成并显着改善他们对肿瘤生长，传染病发病机制和体内基因表达的研究。重要的是，IVIS成像系统将使研究人员能够在不牺牲实验动物的情况下完成体内纵向研究。成像系统可以检测皮下组织中的少至200个细胞，并且可以容易地对麻醉小鼠的肺、淋巴结和骨骼中的细胞的荧光素酶表达进行成像（一次最多10只小鼠）。在小鼠中对人类癌症的转化研究通常受到体内测量或成像肿瘤的能力的限制。IVIS成像系统将允许精确测量皮下和内脏肿瘤中的活肿瘤细胞、对治疗的反应，并大大缩短研究体内人类癌症的发病机制和治疗所需的时间和动物数量。此外，成像系统将使研究人员能够监测体内的基因表达和病原体。这项技术将显著提高研究人员完成目前资助的研究项目的能力，并扩展研究人员提出需要在小鼠或其他小动物体内成像的新转化研究项目的能力。在俄亥俄州州立大学没有类似的仪器，并且在俄亥俄州只有一个IVIS成像系统（在Procter & Gamble Co.，辛辛那提）。该仪器将在研究科学家主管（专门从事成像）和实验室动物兽医的监督下饲养在兽医学院的屏障动物设施（Sisson Hall）中。主要研究者在斯坦福大学的Chris Contag博士（IVIS系统的开发者和Xenogen Corp.的创始人）的实验室接受了IVIS成像系统的使用培训。主要研究者和支持研究科学家和实验动物兽医将对用户进行培训。该软件将在联网计算机上的Windows环境中运行，使调查人员能够从远程站点访问数据。每小时的使用费将为服务合同和主管的继续教育提供资金。将通过兽医生物科学部成像服务的当前系统进行计费。仪器的空间和公用设施以及主管的工资和福利将由兽医生物科学系和兽医学院提供。

DESCRIPTION (provided by applicant): An IVIS Imaging System made by Xenogen Corporation is requested in this Shared Instrument Grant application. The IVIS Imaging System uses a highly efficient CCD camera (in a light-free imaging chamber) and Livinglmage. Software to rapidly capture light emitted from luciferase to image human or animal cells, bacteria, viruses, or genes that are linked to luciferase expression. The instrument will allow fourteen NIH-funded investigators to complete and significantly improve their studies on tumor growth, pathogenesis of infectious disease, and gene expression in vivo using animal models of human disease. Importantly, the IVIS Imaging System will enable the investigators to complete longitudinal studies in vivo without sacrifice of experimental animals. The Imaging System can detect as few as 200 cells in the subcutis and can readily image luciferase expression by cells in lungs, lymph nodes, and bones of anesthetized mice (up to 10 mice at one time). Translational studies on human cancer in mice are usually limited by the ability to measure or image tumors in vivo. The IVIS Imaging System will permit accurate measurement of viable tumor cells in subcutaneous and visceral neoplasms, response to therapy, and greatly shorten the time and animals numbers needed to investigate the pathogenesis and treatment of human cancer in vivo. In addition, the Imaging System will allow investigators to monitor gene expression and pathogenic organisms in vivo. This technology will significantly improve the ability of the investigators to complete currently funded research projects and extend the capabilities of the investigators to propose new translational research projects that require in vivo imaging in mice or other small animals. There is no similar instrument at The Ohio State University, and only one IVIS Imaging System exists in Ohio (at Procter & Gamble Co., Cincinnati). The instrument will be housed in a barrier animal facility (Sisson Hall) in the College of Veterinary Medicine under the supervision of a Research Scientist Supervisor (that specializes in imaging) and a Laboratory Animal Veterinarian. The principal investigator has received training in use of the IVIS Imaging System in the laboratory of Chris Contag, PhD at Stanford University (developer of the IVIS System and founder of Xenogen Corp.). Users will be trained by the Principal Investigator and the supporting Research Scientist and Laboratory Animal Veterinarian. The software will operate in a Windows environment on a networked computer that will allow the investigators to access the data from distant sites. An hourly user fee will provide funds for a service contract and continuing education of the supervisor. Billing will be performed by the current system of the Imaging Services in the Department of Veterinary Biosciences. Space and utilities for the instrument plus salary and benefits for the supervisor will be provided by the Department of Veterinary Biosciences and the College of Veterinary Medicine.