Core--Mouse Pathobiology

核心--小鼠病理学

• 批准号: 6995155
• 负责人: THOMAS J ROSOL
• 金额: $ 14.46万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6995155
• 关键词: animal care; biomedical facility; breast neoplasms; histopathology; imaging /visualization /scanning; laboratory mouse; metastasis; neoplasm /cancer transplantation; oncology

The Mouse Pathobiology Core will be established to provide the necessary facilities and faculty and staff expertise to support all three research projects. A major goal of the Core will be to provide expertise in mouse pathology with up-to-date proficiency in mammary gland and breast cancer pathology and the pathobiology of metastasis. Pathology support will be provided by veterinary anatomic and clinical pathologists that are Diplomats of the American College of Veterinary Pathologists. Expertise in comparative mammary gland pathology will enable appropriate interpretation and evaluation of the mouse models in the research projects and comparison to human mammary cancer. In addition, a D.V.M. postdoctoral trainee in mouse pathology will perform the mouse necropsies (autopsies), cutting and processing of tissues and evaluation of histopathology under the supervision of faculty pathologists. This will provide excellence and continuity of service for the research projects. Other services that will be available to investigators include (1) mouse clinical pathology, including a full-service hematology and clinical chemistry laboratory in the Veterinary Medical Teaching Hospital that can appropriately analyze samples from all species including mice; (2) expertise in mouse imaging, including Faxitron high resolution radiography, scintigraphy, and whole animal or organ bioluminescent and fluorescence imaging; (3) support for studies on metastasis including orthotopic implantation of tumor tissue into the mammary glands or lungs and injection of tumor cells into the left cardiac ventricle to induce bone metastasis; and (4) support for transplantation of mammary gland anlage between mice with different genetic alterations. Faculty for the Mouse Pathobiology Core will be organized from the Department of Veterinary Biosciences. Facilities will include four necropsy rooms (including one devoted to the Core) and an automated Dako immunostainer, microcopy facilities including a 10-headed microscope with video output for conferences, dedicated bioluminescent and fluorescent in vivo imaging instrumentation, digital gross and microscopic photography facilities, histomorphometry equipment including a fluorescent microscope and Bioquant Nova analysis software, mouse surgery and radiology facilities including high resolution scintigraphy in the Colleges of Veterinary Medicine and Medicine. These faculty and services will provide a unique resource to the research projects and significantly enhance the quality and validity of the research data.

将建立小鼠病理生物学核心，以提供必要的设施和教职员工专业知识，以支持所有三个研究项目。核心的主要目标是提供小鼠病理学专业知识，具有乳腺和乳腺癌病理学以及转移病理学的最新水平。兽医解剖学和临床病理学家将提供病理支持，这些病理学家是美国兽医病理学家学院的外交官。比较乳腺病理学的专业知识将在研究项目中对鼠标模型进行适当的解释和评估 并与人类乳腺癌进行比较。此外，D.V.M.小鼠病理学的博士后学员将在教师病理学家的监督下进行小鼠死灵（尸检），组织切割和加工以及组织病理学的评估。这将为研究项目提供卓越的服务。调查人员将使用的其他服务包括（1）小鼠临床病理，包括兽医教学医院的全方位服务血液学和临床化学实验室，可以适当地分析包括小鼠在内的所有物种的样本； （2）小鼠成像方面的专业知识，包括Faxitron高分辨率射线照相，闪烁显像以及整个动物或器官生物发光和荧光成像； （3）支持对转移的研究，包括将肿瘤组织的原位植入到乳腺或肺部，并将肿瘤细胞注射到左心室中，以诱导骨转移； （4）支持具有不同遗传改变的小鼠之间乳腺的移植。小鼠病理生物学核心的教师将由兽医生物科学系组织。设施将包括四个尸检室（包括一个专门介绍核心）和一个自动化的Dako免疫染色器，微型副本设施，包括10头显微镜，包括会议的视频输出，包括专用的生物发光和荧光In in Vivo In in Vivo Image Imagination Instrument Instrumition仪器，数字式和显微镜摄影设施，均匀的型号，均匀的零件，型号的型号，均匀的零件型，型号型号，均匀的设备。手术和放射学设施，包括兽医和医学学院的高分辨率闪烁显像。这些教师和服务将为研究项目提供独特的资源，并显着提高研究数据的质量和有效性。