Core--Mouse Pathobiology

将建立小鼠病理生物学核心，以提供必要的设施和教师和工作人员的专业知识，以支持所有三个研究项目。核心的一个主要目标是提供小鼠病理学方面的专业知识，并掌握乳腺癌和乳腺癌病理学以及转移的病理生物学方面的最新知识。病理学支持将由兽医解剖学和临床病理学家提供，他们是美国兽医病理学家学院的文凭。比较乳腺病理学方面的专业知识将使研究项目中的小鼠模型得到适当的解释和评价 与人类乳腺癌进行比较。此外，D.V.M.在病理学家的监督下，小鼠病理学博士后实习生将进行小鼠尸检（尸体解剖），组织切割和处理以及组织病理学评价。这将为研究项目提供卓越和连续的服务。研究者可获得的其他服务包括：（1）小鼠临床病理学，包括兽医教学医院的全方位血液学和临床化学实验室，可以适当分析包括小鼠在内的所有物种的样本;（2）小鼠成像方面的专业知识，包括Faxitron高分辨率X射线照相术、荧光照相术以及整个动物或器官的生物发光和荧光成像;（3）支持转移研究，包括将肿瘤组织原位移植到乳腺或肺内，以及将肿瘤细胞注射到左心室内以诱导骨转移;及（4）支持在具有不同遗传改变的小鼠之间移植乳腺原基。小鼠病理生物学核心的教师将由兽医生物科学系组织。设施将包括四个尸检室（包括一台专门用于核心的设备）和一台自动化Dako免疫染色仪，显微镜设备，包括一台10头显微镜（带会议视频输出），专用生物发光和荧光体内成像仪器，数字大体和显微摄影设备，组织形态测量设备，包括荧光显微镜和Bioquant Nova分析软件，在兽医学院和医学院建立了老鼠手术和放射学设施，包括高分辨率的放射照相术。这些教师和服务将为研究项目提供独特的资源，并显着提高研究数据的质量和有效性。

The Mouse Pathobiology Core will be established to provide the necessary facilities and faculty and staff expertise to support all three research projects. A major goal of the Core will be to provide expertise in mouse pathology with up-to-date proficiency in mammary gland and breast cancer pathology and the pathobiology of metastasis. Pathology support will be provided by veterinary anatomic and clinical pathologists that are Diplomats of the American College of Veterinary Pathologists. Expertise in comparative mammary gland pathology will enable appropriate interpretation and evaluation of the mouse models in the research projects and comparison to human mammary cancer. In addition, a D.V.M. postdoctoral trainee in mouse pathology will perform the mouse necropsies (autopsies), cutting and processing of tissues and evaluation of histopathology under the supervision of faculty pathologists. This will provide excellence and continuity of service for the research projects. Other services that will be available to investigators include (1) mouse clinical pathology, including a full-service hematology and clinical chemistry laboratory in the Veterinary Medical Teaching Hospital that can appropriately analyze samples from all species including mice; (2) expertise in mouse imaging, including Faxitron high resolution radiography, scintigraphy, and whole animal or organ bioluminescent and fluorescence imaging; (3) support for studies on metastasis including orthotopic implantation of tumor tissue into the mammary glands or lungs and injection of tumor cells into the left cardiac ventricle to induce bone metastasis; and (4) support for transplantation of mammary gland anlage between mice with different genetic alterations. Faculty for the Mouse Pathobiology Core will be organized from the Department of Veterinary Biosciences. Facilities will include four necropsy rooms (including one devoted to the Core) and an automated Dako immunostainer, microcopy facilities including a 10-headed microscope with video output for conferences, dedicated bioluminescent and fluorescent in vivo imaging instrumentation, digital gross and microscopic photography facilities, histomorphometry equipment including a fluorescent microscope and Bioquant Nova analysis software, mouse surgery and radiology facilities including high resolution scintigraphy in the Colleges of Veterinary Medicine and Medicine. These faculty and services will provide a unique resource to the research projects and significantly enhance the quality and validity of the research data.