Comparative Pathology Core

比较病理学核心

• 批准号: 9091440
• 负责人: THOMAS J ROSOL
• 金额: $ 18.55万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9091440
• 关键词: Antigens; Authorship; Autopsy; Bioinformatics; Biopsy; Biostatistics Core; Cells; Collaborations; Comparative Pathology; Computer software; Computers; Confocal Microscopy; Consensus; Data; Data Analyses; Data Collection; Development; Doctor of Philosophy; Ensure; Epithelial-Stromal Communication; Equipment; Evaluation; Evolution; Experimental Designs; Faculty; Freezing; Genetic Models; Goals; Grant; Head; Health Services Research; Histology; Histopathology; Human; Human Pathology; Human Resources; Image; Imaging Techniques; Immunohistochemistry; In Situ; In Situ Hybridization; Instruction; Laboratories; Laboratory mice; Leadership; Lesion; Link; Malignant Neoplasms; Mammary Neoplasms; Mammary gland; Manuscripts; Medical; Medical Oncologist; Methods; MicroRNAs; Microscope; Microscopic; Microscopy; Molecular; Molecular Target; Monitor; Mus; Ohio; Oncologist; Operative Surgical Procedures; Output; Paraffin Tissue; Pathogenesis; Pathologist; Pathology; Pathology Report; Phase; Phenotype; Photography; Physiological; Positioning Attribute; Postdoctoral Fellow; Procedures; Proteins; Proteome; Protocols documentation; Quality Control; Reporting; Research; Research Project Grants; Resolution; Resource Sharing; Resources; Sampling; Services; Slide; Specialist; Staining method; Stains; Supervision; System; Time; Tissue Banks; Tissue Microarray; Tissue Procurements; Tissue Sample; Tissues; Translations; Ultrasonography; Universities; Woman; Work; Writing; base; design; digital; fluorescence imaging; fluorescence microscope; genetic analysis; high standard; histological stains; human tissue; in vivo; interest; laser capture microdissection; malignant breast neoplasm; meetings; member; molecular pathology; mouse model; outcome forecast; programs; protocol development; research study; statistics; symposium; tissue processing; transcriptome; tumor; tumor microenvironment; tumor progression; web site

PROJECT SUMMARY (See instructions): The Comparative Pathology Core A will provide centralized pathology support for the three scientific projects and represents an evolution from the Mouse Pathobiology Core from the initial grant cycle. A major goal of the Comparative Pathology Core will be to provide expertise in mouse and human pathology with up-to-date proficiency in mammary gland and breast cancer pathology. In addition, a DVM, PhD postdoc in mouse pathology will perform the mouse necropsies (autopsies), cutting and processing of tissues, and evaluation of histopathology and special stains under the supervision of faculty pathologists. This will ensure excellence and continuity of service for the research projects. The Core will have 6 personnel that include three pathologists (one medical and two veterinary), medical oncologist (breast cancer specialist), a pathology fellow, and part-time histology technician. The Comparative Pathology Core is essential to ensure integration of the specific aims and experiments in relation to translation of the mouse genetic models to human breast cancer. The three pathologists and oncologist have a long track record of working together on collaborative projects, and therefore are well positioned to provide support for Core A. In addition, all three pathologists and the oncologist have collaborated with Drs. Ostrowski and Leone leading to shared authorship on manuscripts with high impact on mammary cancer microenvironment and its relationship to mammary cancer progression (1, 2). Facilities will include four necropsy rooms (including one devoted to mouse pathology), tissue processing facilities, histology laboratory with four full-time technicians (one devoted to the Core) and an automated Dako immunostainer. In Situ Molecular Pathology Core Lab with a Ventana staining system and NUANCE PLUS InForm computer-based analyses of co-expression of molecular targets, microscopy facilities including a 10-headed microscope with video output for conferences, laser capture microdissection facility and staff, tissue microarray laboratory, mouse imaging facilities including high resolution ultrasound, digital gross and microscopic photography, histomorphometry equipment including a fluorescent microscope and Image Pro Plus analysis software, and mouse surgery facilities. A SharePoint website will facilitate data transfer and sharing between the cores and project staff. These faculty and services will provide a unique resource to the research projects and significantly enhance the quality and validity of the research data.

项目总结（见说明）： 比较病理学核心A将为三个科学项目提供集中的病理学支持，并代表了从最初的资助周期开始的小鼠病理学核心的演变。比较病理学核心的一个主要目标是提供小鼠和人类病理学方面的专业知识，并掌握最新的乳腺和乳腺癌病理学知识。此外，DVM，博士博士后在小鼠病理学将进行小鼠尸检（尸检），组织的切割和处理，并在教师病理学家的监督下进行组织病理学和特殊染色的评价。这将确保为研究项目提供卓越和连续的服务。核心将有6名人员，其中包括3名 病理学家（一名内科医生和两名兽医）、内科肿瘤学家（乳腺癌专家）、一名病理学研究员和兼职组织学技术员。比较病理学核心是必不可少的，以确保整合的具体目标和实验有关的翻译小鼠遗传模型的人类乳腺癌。这三位病理学家和肿瘤学家在合作项目上有着长期的合作记录，因此能够很好地为核心A提供支持。此外，所有三位病理学家和肿瘤学家都与Ostrowski博士和Leone博士合作，共同撰写了对乳腺癌微环境及其与乳腺癌进展的关系具有高度影响的手稿（1，2）。设施将包括四个尸检室（包括一个专门用于小鼠病理学的尸检室）、组织处理设施、组织学实验室（配备四名全职技术人员（一名专门用于核心））和一台自动化Dako免疫染色机。原位分子病理学核心实验室，配备Ventana染色系统和NUANCE PLUS InForm基于计算机的分子靶点共表达分析， 显微镜设施，包括一台10头显微镜（带视频输出），用于会议，激光捕获显微切割设施和工作人员，组织微阵列实验室，小鼠成像设施，包括高分辨率超声，数字大体和显微摄影，组织形态测量设备，包括 荧光显微镜和Image Pro Plus分析软件，以及小鼠手术设备。一个SharePoint网站将促进核心和项目工作人员之间的数据传输和共享。这些教师和服务将为研究项目提供独特的资源，并显着提高研究数据的质量和有效性。