Detection of carbon monoxide in living cells using surface-functionalised mesoporous materials

使用表面功能化介孔材料检测活细胞中的一氧化碳

• 批准号: 2421742
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2421742
• 关键词: Detection; carbon; monoxide; living; cells

Carbon monoxide (CO) is an endogenous gaseous signalling molecule, and a low dosage has been linked to therapeutic benefits such as response to diseases and inflammation. The aim of the project is to design a highly selective probe capable of sensing CO in biological cells as the major challenges today are poor selectivity and sensitivity. A new family of gated silica-based materials with metal surface units will be developed. Sensing can be achieved through a dramatic colour change and an increase in fluorescence; quench and trap a 'cargo' of fluorophores within the mesoporous silica with metal complexes attached to the surfaces acting as 'gates', which will detach themselves in the presence of CO allowing the fluorophore to be released. This produces a significant fluorescence response on exposure to the low concentrations of CO in cells. Through dose/response testing, the fluorescence observed will be correlated to cellular CO concentrations.

一氧化碳（CO）是一种内源性气体信号分子，低剂量与治疗益处有关，如对疾病和炎症的反应。该项目的目的是设计一种能够检测生物细胞中CO的高选择性探针，因为当今的主要挑战是选择性和灵敏度差。一个新的门控硅基材料与金属表面单元将被开发。传感可以通过显着的颜色变化和荧光的增加来实现;淬灭和捕获中孔二氧化硅内的荧光团的“货物”，其中金属络合物附着在充当“门”的表面上，其将在CO存在下自行分离，从而允许释放荧光团。这在暴露于细胞中的低浓度CO时产生显著的荧光响应。通过剂量/反应测试，观察到的荧光将与细胞CO浓度相关。