Novel, Noninvasive Biomarker of Fruit & Vegetable Intake

水果/蔬菜和慢性疾病的流行病学研究由于缺乏饮食摄入/饮食依从性干预的非侵入性客观生物标志物而受到限制。类胡萝卜素的血液浓度被认为是水果和蔬菜摄入量的最佳生物标志物，但需要静脉穿刺，并且对于大型队列研究和干预试验来说过于昂贵。类胡萝卜素存在于包括皮肤在内的许多人体组织中，皮肤中类胡萝卜素的浓度（来自皮肤活组织检查）已被证明是强烈的

Epidemiologic studies of fruits/vegetables and chronic disease have been limited by the lack of non-invasive objective biomarkers of dietary intake/dietary adherence to interventions. Blood concentrations of carotenoids are considered the best biological markers of fruit and vegetable intake, but require venipuncture and are prohibitively expensive for large cohort studies and intervention trials. Carotenoids are found in many human tissues including skin, and skin concentrations of carotenoids (from dermal biopsies) have been shown to be strongly and significantly correlated with plasma concentrations of the same carotenoids (e.g., beta-carotene, r=0.75). We propose to adapt an existing technology, Raman spectroscopy, for the assessment of the carotenoid status of human tissues in vivo as a biomarker of fruit and vegetable intake. More specifically, we propose to (i) refine our current instrument such as to have a rugged, portable device for rapidly assessing carotenoid status ill human skin in vivo. Once our protype instrument is appropriately refined, we then propose to (ii) evaluate the reproducibility, intra-subject variability, and inter-subject variability in dermal cartenoid levels as estimated by Raman spectroscopy at one time and over time. Upon completion of this aim, our final aim is to (iii) determine the correlation between carotenoid status as assessed by Raman spectroscopy and other measures of carotenoid status including dietary intake of fruits/vegetables and specific carotenoids, plasma carotenoid concentrations and carotenioid concentrations in dermal biopsies. This promising technology is currently being developed/refined for the assessment of carotenoids in human retinal tissue (National Eye Institute); the current proposal would greatly broaden the potential use of this noninvasive technology for cancer epidemiology and prevention studies. Raman spectroscopic technology has tremendous potential as a breakthrough method for rapid screening of carotenoid levels in large populations, and development and validation are critically required to move this technology into human population research and surveillance.