Core--Proteomics

描述(由申请人提供)：蛋白质组学核心设施的目标是为分子毒理学中心的研究人员提供分析蛋白质组学能力。蛋白质组学是对蛋白质组的研究，蛋白质组是基因组的功能补充，由不同的结构和催化功能的多蛋白质机器组成。由于蛋白质是许多化学和物理制剂的主要靶标，蛋白质组学方法对于确定环境因子影响生命系统的分子机制至关重要。分析蛋白质组学集成了蛋白质和多肽分离、质谱分析和生物信息学的工具，以表征蛋白质组及其组成蛋白质。蛋白质组学核心设施位于MS设施内，该设施容纳了范德比尔特的几个研究中心使用的仪器和资源，包括分子毒理学中心。蛋白质组学核心设施服务于几个目标：1)为蛋白质组学研究提供仪器，核心为 蛋白质和多肽分离、用于蛋白质采样和消化的机器人、用于蛋白质和多肽分析的MS仪器和数据系统、用于数据存档和分析的软件和生物信息学工具。2)为了向中心研究人员提供分析蛋白质组学服务，中心将提供常规的分析蛋白质组学服务，特别是蛋白质鉴定、一些蛋白质翻译后修饰的分析以及以快速周转方式进行的基于凝胶的蛋白质组分析。核心还将为核心和中心研究人员之间合作完成的更复杂的蛋白质组学项目提供一个平台。3)为了向中心研究人员提供蛋白质组学分析和实验设计方面的专业知识和培训，核心领导、经理和技术人员将协助中心研究人员及其实验室工作人员设计与蛋白质组相关的实验和分析数据。中心将根据学生、博士后和其他实验室工作人员的需要和兴趣，为他们提供相关技术、仪器和数据分析工具方面的培训。4)为了促进将新技术过渡到中心的蛋白质组学研究，蛋白质组学设施核心的一个独特特点是它位于MS设施内，这将促进 将与质谱学相关的新技术和方法快速整合到中心研究人员的研究项目中。Liebler教授(核心领导者)和Caprioli教授(MS主任和中心调查员)的研究小组正在为蛋白质组学研究开发新的方法和仪器，这些资源可以通过蛋白质组学设施核心向中心研究人员提供。

Description (provided by applicant): The goal of the Proteomics Core Facility is to provide analytical proteomics capabilities to investigators in the Center in Molecular Toxicology. Proteomics is the study of proteomes, which are the functional complements to genomes and which comprise diverse structural and catalytically functional multiprotein machines. Because proteins are principal targets of many chemical and physical agents, proteomic approaches are essential to defining the molecular mechanisms by which environmental agents affect living systems. Analytical proteomics integrates tools for protein and peptide separations, mass spectrometric analysis, and bioinformatics to characterize proteomes and their component proteins. The Proteomics Core Facility is housed within the MS facility, which houses instrumentation and resources used by several research centers at Vanderbilt, including the Center in Molecular Toxicology. The Proteomics Core Facility serves several objectives, 1) To provide instrumentation for proteomics studies, the Core provides instrumentation for protein and peptide separations, robotics for protein sampling and digestion, MS instrumentation for protein and peptide analysis and data systems, software and bioinformatics tools for data archival and analysis. 2) To provide analytical proteomics services to Center investigators, the Core will provide routine analytical proteomics services, particularly protein identification, analysis of some protein post-translational modifications and 2D gel-based proteomics analyses in a rapid-turnaround manner. The Core also will provide a platform for more elaborate proteomics projects to be done in collaboration between the Core and Center investigators. 3) To provide expertise and training in proteomics analysis and experimental design to Center Investigators, the Core Leader, Manager, and technical staff will assist Center investigators and their laboratory staff in the design of proteomics-related experiments and the analysis of data. The Core will offer training in relevant techniques, instrumentation and data analysis tools to students, postdoctorals and other laboratory staff depending on their needs and interests. 4) To facilitate the transition of new technology into proteomics research in the Center, a unique feature of the Proteomics Facility Core is its location within the MS facility, which will facilitate the rapid integration of new mass spectrometry-related technologies and approaches into research projects of Center Investigators. The research groups of Professors Liebler (Core Leader) and Caprioli (Director of MS and a Center investigator) are developing new approaches and instrumentation for proteomics research and these resources can be made available to Center investigators through the Proteomics Facility Core.