Core--Proteomics

描述（由申请人提供）：蛋白质组学核心设施的目标是为分子毒理学中心的研究人员提供分析蛋白质组学的能力。蛋白质组学是对蛋白质组的研究，蛋白质组是基因组的功能补充，由多种结构和催化功能的多蛋白质机器组成。由于蛋白质是许多化学和物理因素的主要目标，蛋白质组学方法对于定义环境因素影响生命系统的分子机制至关重要。分析蛋白质组学集成了蛋白质和肽分离，质谱分析和生物信息学工具，以表征蛋白质组学及其组成蛋白。蛋白质组学核心设施位于MS设施内，该设施包含范德比尔特大学几个研究中心使用的仪器和资源，包括分子毒理学中心。蛋白质组学核心设施有几个目的，1)为蛋白质组学研究提供仪器，核心提供仪器

Description (provided by applicant): The goal of the Proteomics Core Facility is to provide analytical proteomics capabilities to investigators in the Center in Molecular Toxicology. Proteomics is the study of proteomes, which are the functional complements to genomes and which comprise diverse structural and catalytically functional multiprotein machines. Because proteins are principal targets of many chemical and physical agents, proteomic approaches are essential to defining the molecular mechanisms by which environmental agents affect living systems. Analytical proteomics integrates tools for protein and peptide separations, mass spectrometric analysis, and bioinformatics to characterize proteomes and their component proteins. The Proteomics Core Facility is housed within the MS facility, which houses instrumentation and resources used by several research centers at Vanderbilt, including the Center in Molecular Toxicology. The Proteomics Core Facility serves several objectives, 1) To provide instrumentation for proteomics studies, the Core provides instrumentation for protein and peptide separations, robotics for protein sampling and digestion, MS instrumentation for protein and peptide analysis and data systems, software and bioinformatics tools for data archival and analysis. 2) To provide analytical proteomics services to Center investigators, the Core will provide routine analytical proteomics services, particularly protein identification, analysis of some protein post-translational modifications and 2D gel-based proteomics analyses in a rapid-turnaround manner. The Core also will provide a platform for more elaborate proteomics projects to be done in collaboration between the Core and Center investigators. 3) To provide expertise and training in proteomics analysis and experimental design to Center Investigators, the Core Leader, Manager, and technical staff will assist Center investigators and their laboratory staff in the design of proteomics-related experiments and the analysis of data. The Core will offer training in relevant techniques, instrumentation and data analysis tools to students, postdoctorals and other laboratory staff depending on their needs and interests. 4) To facilitate the transition of new technology into proteomics research in the Center, a unique feature of the Proteomics Facility Core is its location within the MS facility, which will facilitate the rapid integration of new mass spectrometry-related technologies and approaches into research projects of Center Investigators. The research groups of Professors Liebler (Core Leader) and Caprioli (Director of MS and a Center investigator) are developing new approaches and instrumentation for proteomics research and these resources can be made available to Center investigators through the Proteomics Facility Core.