Microcoil-based Probe for Protein NMR

用于蛋白质 NMR 的微线圈探针

• 批准号: 6833284
• 负责人: TIMOTHY L PECK
• 金额: $ 9.98万
• 依托单位: PROTASIS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6833284
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; carbon; dielectric property; fluid flow; hydrogen ions; molecular probes; nitrogen; nuclear magnetic resonance spectroscopy; polymers; protein structure; temperature

DESCRIPTION (provided by applicant): The goal of this effort is the creation of a new line of microcoil-based NMR detection probes specifically targeted to protein analysis. The submitting organization will utilize a patented and exclusively licensed approach to susceptibility matching that has enabled small molecule analysis using microcoil-based probes. The fundamental contribution of microcoils to protein analysis is the ability to acquire data using less sample, micrograms rather than milligrams. This provides the protein chemist with an NMR measurement solution that generally saves time by requiring less expressed protein, and that accommodates expression techniques that are of potentially high value but potentially lower yield. The probes developed under this SBIR will be designed to interface to standard NMR spectrometers ranging in (proton) frequency from 400 MHz - 750 MHz, and to sample management and analytical separations technologies both in existence and under development in the rapidly growing field of proteomics. The immediate market will be the pharmaceutical and medicinal chemistry industries.

描述（由申请人提供）：这项工作的目标是创建一个新的基于微线圈的核磁共振检测探针系列，专门针对蛋白质分析。提交组织将利用专利和独家许可的方法进行敏感性匹配，该方法可以使用基于微线圈的探针进行小分子分析。微线圈对蛋白质分析的根本贡献是能够使用更少的样品获得数据，微克而不是毫克。这为蛋白质化学家提供了一种核磁共振测量解决方案，通常通过需要较少表达的蛋白质来节省时间，并且可以适应潜在高价值但潜在低产量的表达技术。根据该SBIR开发的探针将被设计用于连接标准的核磁共振光谱仪（质子）频率范围从400 MHz - 750 MHz，以及在快速增长的蛋白质组学领域中现有和正在开发的样品管理和分析分离技术。直接的市场将是制药和药物化学行业。