Fluoroquinolone Resistance in Streptococcus Pyogenes

我们之前描述了氟喹诺酮耐药是一种在我们实验室从一名NIH患者的血液中分离到的化脓性链球菌。在本研究中，我们将确定来自芝加哥的化脓性链球菌对氟喹诺酮类药物耐药的频率。我们将对这些分离株的gyrA和parC基因进行测序，以确定导致氟喹诺酮耐药的点突变。使用一组抗生素纸片(Kirby-Bauer法)对分离株进行耐药性筛选，并用最小抑菌浓度法(ETest)确认耐药性。在93株菌株中，我们鉴定出10株对氟喹诺酮类药物有不同程度耐药性的菌株。我们目前正在对另一株分离株进行筛选。所有耐药分离株将使用我们之前发表的出版物中描述的引物集进行测序。

We previously described flouroquinolone resistance is an isolate of Streptococcus pyogenes isolated in our laboratory from the blood of an NIH patient. In the current study we will determine the frequency of fluoroquinolone resistance among S. pyogenes isolates from Chicago. We will sequence the gyrA and parC genes of these isolates to determine the point mutations responsible for the fluoroquinolone resistance. Isolates are screened for resistance using a panel of antibiotic disks (Kirby-Bauer method) and the resistance is confirmed with a minimum inhibitory concentration method (eTest). Among 93 isolates we identified 10 isolates with varying degrees of fluoroquinolone resistance. We are currently screening another 89 isolates. All resistant isolates will be sequenced using the primer sets described in our previous publication.