Nanostructured substrates for cell assays

用于细胞分析的纳米结构基质

• 批准号: 6935759
• 负责人: Barbara A. Israel
• 金额: $ 58.65万
• 依托单位: PLATYPUS TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935759
• 关键词: bioassay; bioengineering /biomedical engineering; biotechnology; cell membrane; cell migration; cell sorting; chemical stability; chemical synthesis; cytology; gold; liquid crystal; molecular film; nanotechnology; technology /technique development

DESCRIPTION (provided by applicant): Our goal in this Phase 2 SBIR proposal is to develop and optimize the components for a novel cell migration assay. In Phase 1, we demonstrated that liquid crystals assume a predictable orientation in response to cellular membranes, and therefore they could be used in a sensitive, rapid, generalizable method for reporting cell number. In this application, we propose to build on the ability of liquid crystals to accurately report cell number and spatial location in the development of a quantitative assay for cell migration. This innovative approach will provide a technology with many intrinsic advantages over current methods by reducing labor, time and cost and by providing compatibility with high throughput formats.

描述（由申请人提供）：我们在此阶段2 SBIR提案中的目标是开发和优化新型细胞迁移测定的组件。在阶段1中，我们证明了液晶对细胞膜的响应假设可预测的方向，因此可以将它们用于敏感，快速，可推广的方法来报告细胞数。在此应用中，我们建议建立液晶在开发细胞迁移定量测定中准确报告细胞数和空间位置的能力。这种创新的方法将通过减少劳动力，时间和成本以及提供高通量格式的兼容性来为当前方法提供许多固有优势。