Nanostructured substrates for cell assays

用于细胞分析的纳米结构基质

• 批准号: 6690278
• 负责人: Barbara A. Israel
• 金额: $ 34.74万
• 依托单位: PLATYPUS TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6690278
• 关键词: bioassay; bioengineering /biomedical engineering; biotechnology; cell migration; cell sorting; chemical stability; chemical synthesis; cytology; gold; liquid crystal; molecular film; nanotechnology; technology /technique development

DESCRIPTION (provided by applicant): The long-term goal of this Phase I SBIR is to develop optimized components (nanostructured substrates) for use in the creation of a novel class of rapid, liquid crystal based cellular assays of enumeration, proliferation and function. There is a need for new technologies that are less labor intensive, consume fewer cells and reagents, can be performed in less time, allow for separation of chemotaxis from chemokinesis, provide better control over spatial and temporal aspects of the delivery of concentration gradients and are amenable to high throughput formats. Platypus Technology (tm) can be the foundation for such assays to accelerate basic cell research, drug discovery, and evaluation of anti-cancer drugs. The combination of nanostructured substrates with liquid crystals requires no labels or additional reagents to report the presence of cells. It will provide a unified platform for many diverse cellular assays. In this proposal, we will fabricate substrates with nanoscale topography by three methods: nanoscale molding, abrasion of polymeric materials and oblique deposition of thin films of gold. We will comparatively evaluate the ability of these substrates to: align liquid crystals in the presence of non-specific adsorption of proteins in cell media, support cell functions of epithelial, fibroblast and vascular endothelial cells, report cell numbers, and retain functional stability during a six-month storage period. At the completion of these studies we will have optimized the core component of a new technology for enumeration and functional evaluation of cells.

描述（由申请人提供）：该阶段I SBIR的长期目标是开发优化的组件（纳米结构基板），以创建一种新型的快速，液晶基于枚举，增殖和功能的快速，液晶基蜂窝测定。需要在更少的时间内进行劳动力密集的新技术，消耗较少的细胞和试剂，允许将趋化性与趋化因子分离，从而更好地控制浓度梯度的空间和时间方面，并且可以通过高吞吐量格式进行。鸭嘴兽技术（TM）可以成为加速基本细胞研究，药物发现和评估抗癌药物的基础。纳米结构底物与液晶的组合不需要标签或其他试剂来报告细胞的存在。它将为许多各种蜂窝测定法提供一个统一的平台。在此提案中，我们将通过三种方法来制造具有纳米级地形的底物：纳米级成型，聚合物材料的磨损和薄膜的斜膜。我们将比较评估这些底物在细胞培养基中存在非特异性蛋白质吸附，在六个月的存储期间，在细胞培养基中对蛋白质非特异性吸附，支持细胞，成纤维细胞和血管内皮细胞的细胞功能，报告细胞数量和保持功能稳定性的细胞功能。完成这些研究时，我们将优化一种新技术的核心组成部分，用于列举和功能评估细胞。