Biophotonic Detection of WNV ABS. for Multiple Species

WNV ABS 的生物光子检测。

• 批准号: 6497401
• 负责人: Barbara A. Israel
• 金额: $ 24.76万
• 依托单位: PLATYPUS TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497401
• 关键词: Flaviviridae; West Nile virus; antiviral antibody; communicable disease diagnosis; diagnosis design /evaluation; gold; liquid crystal; nanotechnology; surface coating

DESCRIPTION (provided by applicant):The investigation of an epizootic presents the challenge of detecting antibodies in multiple species. ELISA, the method commonly used for screening large numbers of samples for antibodies, requires species-specific secondary antibodies. In the recent outbreak of West Nile virus in New York City, at least 18 species of birds, as well as horses and humans have been infected with the virus. Platypus technology is based on the exquisite sensitivity of liquid crystals to nanometer scale changes in surface topography of thin films of gold. The binding of antibodies from any species to specific receptors displayed on the surface is reported within seconds by the transduction of visible light through a liquid crystal cell. This platform technology is rapid, sensitive (1000 molecules can be detected), suitable for low technology environments and is adaptable to automation. It will find numerous applications in research, clinical diagnostics, epidemiology, drug discovery and many other areas. We will develop an assay to detect West Nile virus antibodies from any species based on this technology. PROPOSED COMMERCIAL APPLICATION: Platypus technology is a broad-based platform technology that will have application in all fields where the detection of molecular interactions is critical. There is a growing market for rapid viral diagnostics. Our technology can bring diagnosis closer to the point of care. The ability to detect antibodies from multiple species will be of great value to epidemiologic studies.

描述（由申请人提供）：对动物流行病的调查 检测多个物种中的抗体的挑战。酶联免疫吸附测定法 通常用于筛选大量样本中的抗体，需要 物种特异性二抗。在最近爆发的西尼罗河 纽约市至少 18 种鸟类以及马和 人类已经感染了该病毒。 Platypus 技术基于 液晶对表面纳米级变化的灵敏性 金薄膜的形貌。任何物种的抗体与 表面上显示的特定受体在几秒钟内由 通过液晶盒传输可见光。这个平台 技术快速、灵敏（可检测1000个分子），适合 低技术环境并适应自动化。它会发现 在研究、临床诊断、流行病学、药物方面的大量应用 发现和许多其他领域。我们将开发一种检测西尼罗河的方法 基于该技术的任何物种的病毒抗体。 拟议的商业应用： Platypus 技术是一种基础广泛的平台技术，将会有应用 在分子相互作用检测至关重要的所有领域。有一种不断增长的 快速病毒诊断市场。我们的技术可以使诊断更加接近 护理点。检测来自多个物种的抗体的能力将具有巨大的价值 到流行病学研究。