Biophotonic Detection of WNV ABS. for Multiple Species

WNV ABS 的生物光子检测。

• 批准号: 6497401
• 负责人: Barbara A. Israel
• 金额: $ 24.76万
• 依托单位: PLATYPUS TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497401
• 关键词: Flaviviridae; West Nile virus; antiviral antibody; communicable disease diagnosis; diagnosis design /evaluation; gold; liquid crystal; nanotechnology; surface coating

DESCRIPTION (provided by applicant):The investigation of an epizootic presents the challenge of detecting antibodies in multiple species. ELISA, the method commonly used for screening large numbers of samples for antibodies, requires species-specific secondary antibodies. In the recent outbreak of West Nile virus in New York City, at least 18 species of birds, as well as horses and humans have been infected with the virus. Platypus technology is based on the exquisite sensitivity of liquid crystals to nanometer scale changes in surface topography of thin films of gold. The binding of antibodies from any species to specific receptors displayed on the surface is reported within seconds by the transduction of visible light through a liquid crystal cell. This platform technology is rapid, sensitive (1000 molecules can be detected), suitable for low technology environments and is adaptable to automation. It will find numerous applications in research, clinical diagnostics, epidemiology, drug discovery and many other areas. We will develop an assay to detect West Nile virus antibodies from any species based on this technology. PROPOSED COMMERCIAL APPLICATION: Platypus technology is a broad-based platform technology that will have application in all fields where the detection of molecular interactions is critical. There is a growing market for rapid viral diagnostics. Our technology can bring diagnosis closer to the point of care. The ability to detect antibodies from multiple species will be of great value to epidemiologic studies.

描述（由申请人提供）：epizootic Presents的调查 检测多种物种中抗体的挑战。 Elisa，方法 通常用于筛选大量抗体样品，需要 物种特异性的二级抗体。在最近的西尼罗河爆发中 纽约市的病毒，至少18种鸟类以及马和马 人类已被病毒感染。鸭嘴兽技术基于 液晶对纳米尺度表面变化的敏感性 黄金薄膜的地形。从任何物种与 在几秒钟内报告了表面上显示的特定受体 通过液晶细胞转导可见光。这个平台 技术是快速，敏感的（可以检测到1000个分子），适合 低技术环境，可适应自动化。它会发现 在研究，临床诊断，流行病学，药物中的许多应用 发现和许多其他领域。我们将开发一个检测西尼罗河的测定法 基于该技术的任何物种的病毒抗体。 拟议的商业应用： Platypus Technology是一种基于广泛的平台技术，它将具有应用 在所有检测分子相互作用至关重要的领域中。有一个增长 快速病毒诊断的市场。我们的技术可以使诊断更接近 护理点。从多种物种中检测抗体的能力将具有很大的价值 进行流行病学研究。