Rapid Liquid Crystal Detection of Ras

Ras 的快速液晶检测

• 批准号: 6995118
• 负责人: Barbara A. Israel
• 金额: $ 20万
• 依托单位: PLATYPUS TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6995118
• 关键词: biological signal transduction; guanine nucleotide binding protein; liquid crystal; magnetic field; protein quantitation /detection; proteomics; technology /technique development

DESCRIPTION (provided by applicant): We propose to pursue the development of a novel, rapid (<30 min) exquisitely sensitive (femtogram), label-free platform technology using liquid crystals for the quantification of Ras, a key signaling protein. In this proposal, we will develop a method for the rapid capture of Ras from solution onto micro or nano-sized magnetic particles and then detect the captured Ras on nanostructured surfaces using liquid crystals. The approach involves direct detection of Ras, does not require secondary antibodies, and will permit rapid detection of low concentrations of proteins in small sample volumes, such as tumor cell lysates. In Phase 2, we will expand the technology to detection of other signaling proteins as well as simultaneous determination of their state of activation. The elements of this technology are readily adaptable to high throughput systems suitable for the identification and development of therapeutic compounds through an understanding of their action on cell signaling pathways.

描述（由申请人提供）：我们建议使用新型，快速（<30分钟）精心敏感（femtogram），无标签的平台技术，使用液晶来定量RAS（一种关键的信号蛋白）。在此提案中，我们将开发一种方法，以快速捕获RAS从溶液中捕获微或纳米尺寸的磁性颗粒，然后使用液晶检测纳米结构表面上捕获的RAS。该方法涉及直接检测Ras，不需要二抗，并且将允许在小样品体积（例如肿瘤细胞裂解物）中快速检测低浓度的蛋白质。在第2阶段，我们将扩展技术以检测其他信号蛋白，并同时确定其激活状态。该技术的元素很容易适应高通量系统，适用于通过了解其对细胞信号通路的作用来识别和开发治疗化合物的元素。