Rapid Liquid Crystal Detection of Ras

描述（由申请人提供）：我们建议开发一种新型的、快速的（<30分钟）非常灵敏的（飞图）、无标记的平台技术，使用液晶来定量Ras（一种关键的信号蛋白）。在这个提议中，我们将开发一种从溶液中快速捕获Ras到微或纳米级磁性颗粒上的方法，然后使用液晶检测纳米结构表面上捕获的Ras。该方法涉及直接检测Ras，不需要二抗，并且可以快速检测小样本量中的低浓度蛋白质，例如肿瘤细胞裂解物。在第二阶段，我们将把这项技术扩展到检测其他信号蛋白，并同时确定它们的激活状态。通过了解治疗性化合物对细胞信号通路的作用，该技术的元素很容易适应于适合于鉴定和开发治疗性化合物的高通量系统。

DESCRIPTION (provided by applicant): We propose to pursue the development of a novel, rapid (<30 min) exquisitely sensitive (femtogram), label-free platform technology using liquid crystals for the quantification of Ras, a key signaling protein. In this proposal, we will develop a method for the rapid capture of Ras from solution onto micro or nano-sized magnetic particles and then detect the captured Ras on nanostructured surfaces using liquid crystals. The approach involves direct detection of Ras, does not require secondary antibodies, and will permit rapid detection of low concentrations of proteins in small sample volumes, such as tumor cell lysates. In Phase 2, we will expand the technology to detection of other signaling proteins as well as simultaneous determination of their state of activation. The elements of this technology are readily adaptable to high throughput systems suitable for the identification and development of therapeutic compounds through an understanding of their action on cell signaling pathways.