Biophotonic detection of WNV Abs from Multiple Species

多种物种 WNV 抗体的生物光子检测

• 批准号: 7109011
• 负责人: Barbara A. Israel
• 金额: $ 84.69万
• 依托单位: PLATYPUS TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7109011
• 关键词: Flaviviridae; West Nile virus; antiviral antibody; communicable disease diagnosis; diagnosis design /evaluation; gold; liquid crystal; nanotechnology; surface coating

DESCRIPTION (provided by applicant): This Phase 2 proposal will further the development of a fundamentally new, broadly applicable technology (Platypus technology) for the rapid, label-free detection of proteins. In Phase 1, we demonstrated the use of liquid crystals in combination with affinity contact printing for the detection of antibodies from multiple species and of multiple isotypes. In this proposal, we develop alternatives to microscopic and camera dependent methods for analysis of this technology and we transform the technology into a multi-well format, suitable for rapid, customized multiplexing and appropriate for semi-automated high throughput testing. We also demonstrate the generality of the approach for antibody detection and we com pare Platypus Antibody Assay with ELISA and SN detection of antibodies using sera from WNV infected horses and avians.

描述（由申请人提供）：本2期提案将进一步开发一种全新的、广泛适用的技术（鸭嘴兽技术），用于快速、无标记检测蛋白质。在第1阶段，我们展示了使用液晶结合亲和接触印刷检测来自多个物种和多个同种型的抗体。在这项提案中，我们开发了替代显微镜和相机依赖的方法来分析这项技术，我们将该技术转化为多孔格式，适用于快速，定制的多路复用和适合半自动化高通量测试。我们还证明了抗体检测方法的通用性，并将鸭嘴兽抗体检测与使用WNV感染马和鸟类血清进行的抗体ELISA和SN检测进行了帕雷。