权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Diabetes, Exercise and Postprandial Oxidative Stress

糖尿病、运动和餐后氧化应激

基本信息

批准号：
6910627
负责人：
LAURETTA T QUINN
金额：
$ 45.97万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-09-01 至 2007-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6910627
关键词：
aerobic exercise antioxidants clinical research diabetes mellitus therapy diet disease /disorder onset glucose clamp technique glucose metabolism human subject human therapy evaluation lipid metabolism noninsulin dependent diabetes mellitus oxidative stress patient oriented research respiratory function young adult human (21-34)

项目摘要

DESCRIPTION (provided by applicant): Both postprandial oxidative stress and derangements in carbohydrate (CHO) and lipid metabolism have been implicated in the development of complications in type 2 DM. Yet the ability of aerobic exercise to improve these derangements has not been fully explored. This study will examine the effects of an acute bout of aerobic exercise (30 minutes of treadmill walking at 65% VO2Max) in a group of physically untrained subjects with younger-onset type 2 DM and non-DM controls on: (1) CHO and lipid metabolism (glucose, insulin, C-peptides and triglycerides) following 3 meals; (2) postprandial oxidative stress (total MDA, F2 Isoprostanes, total hydroperoxides, vitamin A, E and carotenoids) and LDL susceptibility to oxidation (lag time, maximal conjugated diene formation) following 3 meals; and (3) oxidative stress and LDL susceptibility to oxidation 30 minutes after an acute bout of aerobic exercise. In addition, the relationship between baseline insulin sensitivity, antioxidant status, VO2Max and exercise-induced changes in oxidative stress, CHO and lipid metabolism will be explored. The study will include 35 subjects with type 2 DM (ages 18-40) with duration of diabetes (<7 years) and 35 non-DM controls who will participate in a nonexercise (NE) and an exercise (E) protocols, performed in random order at the General Clinical Research Center. Each of the protocols will be three days in duration. On the first day of either protocol, a glucose clamp will be performed for measurement of insulin sensitivity. On the second day of the E protocol, baseline parameters of oxidative stress, LDL susceptibility to oxidation and lipid metabolism will be obtained prior to an acute bout of aerobic exercise and periodically throughout the post exercise period, during which three meals will be consumed. On the second day of the NE protocol the same protocol will be followed without the acute exercise bout. On the morning of the third day of each protocol (24 hours after the E or NE session), fasting parameters of CHO, lipid and oxidative stress will be drawn. The diet will follow the National Cholesterol Education Program guidelines with 30% fat as energy, with 10:10:10% from saturated, monounsaturated and polyunsaturated fat, respectively with 1/3 of energy requirements consumed at each meal. This study represent a novel approach to the use of aerobic exercise in the treatment of type 2 DM, providing data for future investigations and exercise training studies in this area.

描述(申请人提供)：餐后氧化应激和碳水化合物(CHO)和脂肪代谢的紊乱都与2型糖尿病并发症的发生有关。然而，有氧运动改善这些错乱的能力还没有得到充分的探索。本研究将在一组年轻的2型糖尿病患者和非糖尿病对照组中，观察一次急性有氧运动(在65%最大摄氧量下步行30分钟)对以下方面的影响：(1)三餐后的CHO和脂代谢(葡萄糖、胰岛素、C肽和甘油三酯)；(2)餐后氧化应激(总丙二醛、F2异前列腺素、总过氧化氢、维生素A、E和类胡萝卜素)和三餐后低密度脂蛋白的氧化易感性(滞后时间，最大共轭二烯形成)；以及(3)急性有氧运动30分钟后的氧化应激和低密度脂蛋白氧化易感性。此外，还将探讨基线胰岛素敏感性、抗氧化状态、最大摄氧量与运动引起的氧化应激、CHO和脂代谢变化之间的关系。这项研究将包括35名2型糖尿病患者(年龄18-40岁)和35名非糖尿病对照组，他们将参加在综合临床研究中心按随机顺序进行的非运动(NE)和运动(E)方案。每项协议的期限为三天。在任一方案的第一天，将进行葡萄糖钳夹以测量胰岛素敏感性。在E方案的第二天，将在一次急性有氧运动之前和在运动后的整个运动期内定期获得氧化应激、低密度脂蛋白氧化敏感性和脂肪代谢的基线参数，在此期间将消耗三餐。在NE方案的第二天，将遵循相同的方案，不进行急性运动比赛。在每个方案的第三天早上(E或NE会议后24小时)，抽取空腹CHO、血脂和氧化应激参数。饮食将遵循国家胆固醇教育计划指南，以30%的脂肪为能量，饱和脂肪、单不饱和脂肪和多不饱和脂肪分别为10：10：10%，每餐消耗三分之一的能量需求。本研究为有氧运动在2型糖尿病治疗中的应用提供了一种新的方法，为该领域未来的研究和运动训练研究提供了数据。