Core--Embryonic stem cell engineering

核心--胚胎干细胞工程

• 批准号: 7116676
• 负责人: ALEX L KOLODKIN
• 金额: $ 25.35万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7116676
• 关键词: biomedical facility; biotechnology; embryonic stem cell; gene targeting; genetically modified animals; laboratory mouse; tissue /cell culture

The aim of the Embryonic Stem (ES) Cell Engineering facility is to accelerate research programs that depend on the development of genetically altered mouse models (knock-outs, knock-ins, and inducible knock-outs) that require ES cell manipulation. Successful ES cell engineering is a major hurdle for the realization of research goals for many NINDS investigators at JHU SOM (see below), and is currently performed by individual laboratories with varying degrees of success. The ES Cell Engineering Core will implement cutting-edge approaches for ES cell manipulation to assure that investigators use the most efficient strategies to generate animal models for NINDS supported research. These strategies will include generation of ES cells from existing mouse mutant lines for subsequent gene targeting and chimeric embryo production, and multi-hit transgenic targeting protocols that use novel recombination approaches for controlled site-directed mutagenesis. By establishing ES cell engineering as a core resource we will standardize protocols from the most successful laboratories, markedly increase the efficiency of model generation, and speed the pace of NINDS-supported research at JHU SOM. There is currently no core facility at JHU SOM that can perform ES cell engineering. The ES Cell Engineering Core will work in synergy with the current Transgenic Animal facility at JHU SOM, which provides complementary services such as injection of ES cells into blastocysts and animal husbandry necessary to achieve high chimera mice. There will be no overlap in the services provided by the ES Cell Engineering Core and the Transgenic Animal facility.

胚胎干细胞(ES)细胞工程设施的目标是加快依赖于开发需要ES细胞操作的转基因小鼠模型(敲除、敲入和诱导敲除)的研究计划。成功的胚胎干细胞工程是JHU SOM许多NINDS研究人员实现研究目标的主要障碍(见下文)，目前由各个实验室进行，取得了不同程度的成功。胚胎干细胞工程核心将实施胚胎干细胞操作的尖端方法，以确保研究人员使用最有效的策略来为NINDS支持的研究生成动物模型。这些策略将包括从现有的小鼠突变系中产生ES细胞，用于随后的基因打靶和嵌合胚胎生产，以及多次打击 转基因靶向协议，使用新的重组方法进行受控的定点突变。通过将胚胎干细胞工程作为核心资源，我们将标准化来自最成功实验室的方案，显著提高模型生成的效率，并加快JHU SOM由NINDS支持的研究的步伐。目前，JHU SOM没有能够进行胚胎干细胞工程的核心设施。胚胎干细胞工程核心将与JHU SOM目前的转基因动物设施协同工作， 它提供补充服务，如将ES细胞注射到囊胚中，并进行必要的畜牧业，以获得高嵌合体小鼠。胚胎干细胞工程核心和转基因动物设施提供的服务不会有重叠。