JHU Center for Neuroscience Research

摘要：JHU神经科学研究中心 在这次修订后的竞争续签中，我们的“JHU神经科学研究中心”NINDS P30中心拨款， 我们建议维护和扩展约翰·霍普金斯大学的两个科学核心设施 在过去的10年里一直支撑着这个中心的医学院：多光子 成像(MPI)核心和小鼠突变核心(MMC；正式名称为ES细胞工程核心)。这些 科学核心目前提供了关键的研究能力和资源，这对 神经科学研究人员自己提供，但这大大加强了个人研究的努力 程序。这些核心提供的实验机会和技术服务是相辅相成的，但确实 不是重复的，NINDS资助的JHU SOM调查人员可以使用的其他设施。中心核心的使用将 继续受益于NINDS资助的12个研究项目，这些项目构成了 该中心，以及JHU SOM的其他NINDS资助和非NINDS资助的调查人员。这项研究 初级中心调查人员的计划解决了一系列重要的悬而未决的问题，这些问题是 NINDS任务。这些包括对兴奋性突触动力学的基础神经科学研究和 功能，海马区细胞回路的组织，皮层回路的定义 躯体感觉，成人神经发生过程中局部回路的建立，细胞和分子 钠通道调节中的痛痒机制和钙信号转导。临床相关工作 包括中风、帕金森病、肌萎缩侧索硬化症的遗传学和分子基础 硬化症、自闭症谱系障碍和睡眠。中心核心的独特能力将继续服务于 作为JHU NINDS资助的调查人员的重要资源，通过扩展 个人调查人员，并作为传播和最新技术实践培训的协调中心 研究方法。通过这些努力，JHU神经科学研究中心将帮助建立 研究健康和疾病中的大脑功能的新策略将造福于更广泛的神经科学 社区。

SUMMARY: JHU Center for Neuroscience Research In this revised competitive renewal of our “JHU Center for Neuroscience Research” NINDS P30 center grant, we propose to both maintain and expand two of the Scientific Core facilities at the Johns Hopkins University School of Medicine (JHU SOM) that have anchored this Center for the past 10 years: the Multiphoton Imaging (MPI) Core and the Murine Mutagenesis Core (MMC; formally “ES Cell Engineering Core”). These Scientific Cores currently provide critical research capabilities and resources that are impractical for neuroscience investigators to provide on their own but that greatly enhance efforts of individual research programs. The experimental opportunities and technical services offered by these Cores complement, but do not duplicate, other facilities available to NINDS-funded investigators at JHU SOM. Use of Center Cores will continue to benefit the 12 NINDS-funded research programs that constitute the Primary Center Investigators of this Center, and also other NINDS-funded and non-NINDS-funded investigators at JHU SOM. The research programs of the Primary Center Investigators address a wide range of significant unsolved questions central to the NINDS mission. These include basic neuroscience investigation into excitatory synapse dynamics and function, the organization of hippocampal place cell circuitry, definition of cortical circuits underlying somatosensation, establishment of local circuitry during adult neurogenesis, cellular and molecular mechanisms of pain and itch, and Ca++ signaling in Na channel regulation. Clinically relevant work encompasses the genetics and molecular underpinnings of stroke, Parkinson's Disease, amyotrophic lateral sclerosis, autism spectrum disorders, and sleep. The unique capabilities of Center Cores will continue to serve as a crucial resource for NINDS-funded investigators at JHU by extending the research capabilities of individual investigators and by serving as a focal point for dissemination and practical training in state-of-the art research approaches. Through these efforts, the JHU Center for Neuroscience Research will help establish new strategies for studying brain function in health and disease that will benefit the broader neuroscience community.