Natural variation in Drosophila female postmating traits

果蝇雌性交配后性状的自然变异

• 批准号: 6925655
• 负责人: DAVID J BEGUN
• 金额: $ 26.91万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6925655
• 关键词: Drosophilidae; animal breeding; animal population genetics; arthropod genetics; behavioral /social science research tag; ethology; evolution; female; fertility; gene expression; genetic screening; genotype; male; polymerase chain reaction; quantitative trait loci; reproductive system; sex behavior; sperm analysis

DESCRIPTION (provided by applicant): In flies, as in many other animals, mating does not guarantee successful reproduction. Postmating prefertilization interactions between males and females (e.g., sperm competition, sperm storage by females) also play a central role in reproduction. Moreover, post-copulatory interactions may have broad evolutionary implications. For example, postmating sexual selection may contribute to the evolution of reproductive isolation. Most research on post-copulatory pre-fertilization biology in flies has focused on male-expressed proteins such as seminal fluid proteins. Though it is well known that female flies are not simply passive vessels and that female-mediated interactions with male products are important to reproductive success, little is known about the genetics or population genetics of female postcopulatory biology. The proposed research aims to begin filling this basic gap in our understanding of Drosophila females. The specific aims of the proposed work are to i) use molecular approaches to identify candidate genes potentially affecting female postcopulatory phenotypes in D. melanogaster, ii) use QTL analysis of recombinant inbred lines of D. melanogaster to identify regions of the genome harboring natural variants affecting female post-copulatory phenotypes, iii) use molecular population genetics to test hypotheses on the causes of variation in candidate genes, iv) investigate divergence of female postcopulatory phenotypes and evolution of postcopulatory incompatibilities in the melanogaster group.