QTL Analysis in Combined Inbred Line Crosses

组合自交系杂交中的QTL分析

• 批准号: 6864850
• 负责人: Gary A Churchill
• 金额: $ 33.8万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6864850
• 关键词: analytical method; biotechnology; computer program /software; computer system design /evaluation; genetic mapping; genetic registry /resource /referral center; inbreeding; information retrieval; meta analysis; method development; phenotype; quantitative trait loci; single nucleotide polymorphism; statistics /biometry

DESCRIPTION (provided by applicant): Rodent inbred line crosses are widely used to map genetic loci associated with complex traits. This approach has proven to be powerful for detecting quantitative trait loci (QTL) however the resolution of QTL locations is often not sufficient to identify the gene (or genes) that are involved. We propose to develop analytical methods to combine information available in two or more inbred line crosses that will help to narrow conventional confidence intervals for QTL regions. Furthermore, by exploiting high-density single nucleotide polymorphism (SNP) data that are currently being generated for the common strains of inbred mice, we expect to achieve gene level resolution of QTLs by combining data from multiple crosses. Our strategy is based on the hypothesis that the common mouse strains are derived from a limited ancestral gene pool and thus QTLs detected in multiple crosses are likely to represent shared ancestral polymorphisms.

描述（由申请人提供）： 啮齿动物近交系杂交被广泛用于绘制与复杂性状相关的遗传位点。这种方法已被证明是强大的检测数量性状基因座（QTL），但QTL位置的分辨率往往是不够的，以确定所涉及的基因（或基因）。我们建议开发分析方法，结合联合收割机的信息，在两个或两个以上的自交系杂交，这将有助于缩小传统的QTL区域的置信区间。此外，通过利用高密度单核苷酸多态性（SNP）数据，目前正在产生的常见品系的近交系小鼠，我们希望实现基因水平的QTL的分辨率结合数据从多个杂交。我们的策略是基于这样的假设，即常见的小鼠品系来自有限的祖先基因库，因此在多个杂交中检测到的QTL可能代表共享的祖先多态性。