Stereology & Live Cell Imaging Workstation

体视学

• 批准号: 6730786
• 负责人: ANDA N. CORNEA
• 金额: $ 21.48万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730786
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cell morphology; fluorescence microscopy; fluorescence resonance energy transfer; fluorescent dye /probe; imaging /visualization /scanning; time resolved data

DESCRIPTION (provided by applicant): This application requests funds for purchasing an imaging workstation that enables stereological measurements based on multichannel fluorescence identification of features to be counted, time-lapse live cell fluorescence imaging and measuring Fluorescence Resonance Energy Transfer (FRET) between cyan and yellow fluorescent proteins (CFP and YFP). Morphometric measurements, such as numbers and sizes of cells, density of neuronal fibers in brain circuits, density of synapses in specific areas or on specific cells, number and stages of ovarian follicles are important determinants of physiological changes associated with conditions that constitute the focus of research of multiple NIH-funded grants at the Oregon National Primate Research Center (ONPRC). Estimating these parameters based on two-dimensional microscopic images is very laborious and is affected by biases, if sampling and analysis techniques of modern stereology are not followed. A stereology compatible imaging system is therefore necessary for the research of our investigators. For other projects, use of green fluorescent proteins and their mutants has created opportunities for identifying the intracellular location, translocation and function of genes of interest. A particularly attractive application is the visualization and measurement of the interaction between biomolecules in physiologically relevant states, in real time, using FRET, especially between CFP and YFP. This is not possible with the instruments currently available to us, but necessary for our NIH-funded investigators. This workstation will be part of the Imaging and Morphology Core of ONPRC located on the West campus of the Oregon Health & Sciences University and will enable the core to provide services that are either not possible now, or that will be significantly improved in accuracy and speed. An internal advisory committee will establish procedures and oversee the general operation of the imaging workstation. The principal investigator will be in charge of the installation, training, scheduling, use and maintenance of the new imaging workstation. The institution is committed to maintenance of instrument and providing oversight, support for staff salary and training.

描述(由申请人提供)：本申请申请资金用于购买成像工作站，该工作站能够基于待计数特征的多通道荧光识别进行立体测量、延时活细胞荧光成像以及测量青色和黄色荧光蛋白(CFP和YFP)之间的荧光共振能量转移(FRET)。形态测量，如细胞的数量和大小，脑回路中神经纤维的密度，特定区域或特定细胞上的突触密度，卵巢卵泡的数量和阶段，是与条件相关的生理变化的重要决定因素，这些条件构成了俄勒冈州国家灵长类动物研究中心(ONPRC)多项NIH资助资助的研究重点。如果不遵循现代体视学的采样和分析技术，基于二维显微图像估计这些参数是非常费力的，并且受到偏差的影响。因此，一个体视学兼容的成像系统对于我们研究人员的研究是必要的。对于其他项目，绿色荧光蛋白及其突变体的使用创造了识别感兴趣基因的细胞内位置、易位和功能的机会。一个特别吸引人的应用是可视化和测量相互作用 生物分子的生理相关状态，实时使用FRET，特别是在CFP和YFP之间。对于我们目前可用的工具来说，这是不可能的，但对于我们NIH资助的调查人员来说，这是必要的。该工作站将是位于俄勒冈健康与科学大学西校区的ONPRC成像和形态核心的一部分，将使核心能够提供现在不可能提供的服务，或者将在精度和速度方面得到显著提高的服务。一个内部咨询委员会将制定程序并监督成像工作站的一般运作。首席调查员将负责新成像工作站的安装、培训、调度、使用和维护。该机构致力于维护工具，并为工作人员的工资和培训提供监督、支持。