Laser Scanning Confocal Microscope

描述（由申请人提供）：这是一份购买徕卡TCS SP5共聚焦系统的资金申请，以取代俄勒冈国家灵长类动物研究中心（ONPRC） 11年历史的类似仪器。ONPRC的成像和形态学核心（Imaging and Morphology Core）是一个区域性的显微镜资源，仅围绕着它建立，现在失败了，共聚焦，迫切需要取代它。在其使用寿命期间，现有的徕卡TCS SP已被大量研究人员大量使用，已产生至少100篇出版物，多个封面，已促成了授予的资助数量和质量的提高，已用于培训至少75名科学家，许多研究生，博士后研究员和国际访问科学家。现在，这个旧的共聚焦系统已经变得不可靠和无法使用，而许多拨款都依赖于通过它获得的数据。没有类似的工具可以满足此应用程序的10个参与者的时间要求。在评估了几个品牌后，我们选择了另一个徕卡，因为它提供了无缝的过渡，因为它与旧的相似，我们对它的性能印象深刻。所要求的功能几乎完全复制了现有的功能，说明了技术的一些进步。我们的研究旨在了解发育、青春期、性功能和衰老的基本机制，并寻找各种疾病的原因和治疗方法，如：肥胖、糖尿病、饮食引起的代谢疾病、多发性硬化症和其他退行性疾病、抑郁症、焦虑、促性腺功能减退症、Rett综合征、尼古丁引起的肺癌。我们的使命是不仅为ONPRC的科学家，而且为整个地区提供培训、专业知识和仪器。一个新的，功能齐全的，最先进的共聚焦是我们完成这一任务的必要条件。

DESCRIPTION (provided by applicant): This is a request for funds to purchase a Leica TCS SP5 confocal system to replace an 11 year old similar instrument at the Oregon National Primate Research Center (ONPRC). The Imaging and Morphology Core at ONPRC, a regional microscopy resource, was built around this only, now failing, confocal and urgently needs to replace it. During its useful lifetime, the existing Leica TCS SP has been heavily used by a large number of investigators, has resulted in at least 100 publications, several covers, has contributed to an increase in the number and quality of grants awarded, has been used to train at least 75 scientists, many graduate students, postdoctoral fellows and international visiting scientists. Now this old confocal has become unreliable and unserviceable, while many grants depend on data acquired with its help. There is no similar instrument that could accommodate the time requirements of the 10 participants to this application. After evaluating several brands, we selected another Leica because it provides a seamless transition, due to the similarity with the old one, and we were impressed by its performance. The features requested almost entirely reproduce the existing ones, accounting for some progress in the technology. The research we serve aims to understand basic mechanisms involved in development, puberty, sexual function and aging, and to find causes and cures for various diseases such as: obesity, diabetes, diet induced metabolic diseases, multiple sclerosis and other degenerative diseases, depression, anxiety, hypogonadotropic hypogonadism, Rett Syndrome, nicotine-induced lung cancer. Our mission is to provide training, expertise and instrument access not only to ONPRC scientists but to the entire region. A new, functional, state of the art confocal is a necessity for us to fulfill this mission.