Laser Scanning Confocal Microscope

描述（由申请人提供）：这是一份资金申请，用于购买Leica TCS SP5共聚焦系统，以替换俄勒冈州国家灵长类动物研究中心（ONPRC）使用了11年的类似仪器。ONPRC的成像和形态学核心是一个区域性显微镜资源，它是围绕这一唯一的，现在失败的共聚焦而建立的，迫切需要更换它。在其使用寿命期间，现有的Leica TCS SP已被大量研究人员大量使用，已导致至少100篇出版物，几个封面，有助于增加授予的赠款的数量和质量，已用于培训至少75名科学家，许多研究生，博士后研究员和国际访问科学家。现在，这种旧的共焦已经变得不可靠和无法使用，而许多赠款依赖于在其帮助下获得的数据。没有任何类似的文书能够满足这一申请的10个参与者的时间要求。在评估了几个品牌后，我们选择了另一个徕卡，因为它提供了一个无缝的过渡，由于与旧的相似，我们对它的性能印象深刻。所要求的功能几乎完全复制了现有的功能，说明了技术上的一些进步。我们所服务的研究旨在了解发育、青春期、性功能和衰老的基本机制，并找到各种疾病的原因和治疗方法，如：肥胖、糖尿病、饮食诱导的代谢疾病、多发性硬化症和其他退行性疾病、抑郁症、焦虑症、低促性腺激素性功能减退症、Rett综合征、尼古丁诱导的肺癌。我们的使命是不仅为ONPRC科学家而且为整个地区提供培训、专业知识和仪器。一个新的，功能性的，最先进的共聚焦是我们完成这一使命的必要条件。

DESCRIPTION (provided by applicant): This is a request for funds to purchase a Leica TCS SP5 confocal system to replace an 11 year old similar instrument at the Oregon National Primate Research Center (ONPRC). The Imaging and Morphology Core at ONPRC, a regional microscopy resource, was built around this only, now failing, confocal and urgently needs to replace it. During its useful lifetime, the existing Leica TCS SP has been heavily used by a large number of investigators, has resulted in at least 100 publications, several covers, has contributed to an increase in the number and quality of grants awarded, has been used to train at least 75 scientists, many graduate students, postdoctoral fellows and international visiting scientists. Now this old confocal has become unreliable and unserviceable, while many grants depend on data acquired with its help. There is no similar instrument that could accommodate the time requirements of the 10 participants to this application. After evaluating several brands, we selected another Leica because it provides a seamless transition, due to the similarity with the old one, and we were impressed by its performance. The features requested almost entirely reproduce the existing ones, accounting for some progress in the technology. The research we serve aims to understand basic mechanisms involved in development, puberty, sexual function and aging, and to find causes and cures for various diseases such as: obesity, diabetes, diet induced metabolic diseases, multiple sclerosis and other degenerative diseases, depression, anxiety, hypogonadotropic hypogonadism, Rett Syndrome, nicotine-induced lung cancer. Our mission is to provide training, expertise and instrument access not only to ONPRC scientists but to the entire region. A new, functional, state of the art confocal is a necessity for us to fulfill this mission.