QUANTITATIVE IMAGING: MORPHOMETRY ANALYSIS OF RHESUS FETAL LUNG

定量成像：恒河猴胎肺的形态学分析

• 批准号: 7348958
• 负责人: ANDA N. CORNEA
• 金额: $ 15.35万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7348958
• 关键词: QUANTITATIVE; IMAGING; MORPHOMETRY; ANALYSIS; RHESUS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The general goal of this project is to introduce modern stereology methods to quantitative imaging projects at ONPRC. Using microscopy to obtain quantitative rather than descriptive information relies on the understanding and implementation of 3D geometry, statistics and sampling theory concepts, not commonly used in biological applications. We are collaborating with Dr. Dallas Hyde from the CNPRC, vice-president of the International Society for Stereology, to standardize protocols for the quantitative analysis of nonhuman primate lung morphometry. Based on Dr. Hyde's experience with standard measurements on adult lungs, we will develop protocols for processing, sampling, imaging and analyzing rhesus fetal lungs for projects currently in work at ONPRC. There are two general aims to this project: 1) to design protocols for processing, dividing and sampling the lung so that it can provide several investigators with complete representation of the whole organ, to be used for several methods. This will have a two-fold advantage: it will increase the efficiency or organ use and would ensure that each study has an adequate representation of the whole organ, and 2) to develop, test, implement and make available to interested ONPRC investigators protocols for measuring various structural parameters of interest in fetal lung. Stereology methods, sometimes tedious and mathematically intimidating will be presented as easy to follow, simple protocols. This initial experience obtained in lung will be then expanded to other organs of interest, starting with pancreas, ovaries and brain regions.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该项目的总体目标是将现代立体学方法引入ONPRC的定量成像项目。使用显微镜获得定量而非描述性信息依赖于对三维几何、统计学和抽样理论概念的理解和实施，而这些概念在生物学应用中并不常用。我们正在与国际立体学学会副主席、CNPRC的Dallas Hyde博士合作，对非人类灵长类动物肺形态计量学的定量分析方案进行标准化。基于Hyde博士在成人肺标准测量方面的经验，我们将为ONPRC目前正在进行的项目制定处理、采样、成像和分析恒河猴胎儿肺的方案。这个项目有两个总体目标：1)设计处理、划分和采样肺的方案，以便它可以为几个研究人员提供整个器官的完整代表，用于几种方法。这将具有双重优势：它将提高器官使用的效率，并确保每项研究都充分代表整个器官；2)开发、测试、实施并向感兴趣的ONPRC研究者提供测量胎儿肺各种结构参数的方案。立体学方法，有时单调乏味，数学上令人生畏，将被呈现为易于遵循的简单协议。在肺部获得的初步经验将扩展到其他感兴趣的器官，从胰腺、卵巢和大脑区域开始。