IMAGING AND MORPHOLOGY CORE

成像和形态学核心

• 批准号: 8125615
• 负责人: ANDA N. CORNEA
• 金额: $ 6.51万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8125615
• 关键词: Avidin; Biotin; Cells; Color; Complex; Confocal Microscopy; Consult; Cornea; Development; Equipment; Fluorescence; Freezing; Gene Expression; Histocytochemistry; Image; Imaging technology; In Situ Hybridization; Label; Laboratories; Lasers; Methods; Microdissection; Microscope; Microscopy; Microtome - medical device; Morphology; Paraffin Tissue; Population; Procedures; Process; Proteomics; Protocols documentation; Research Personnel; Resolution; Scientist; Slide; Staining method; Stains; System; Techniques; Tissue Sample; Tissues; Training; cRNA Probes; cryostat; digital imaging; image processing; investigator training; laser capture microdissection; meetings; tissue processing

The objective of the Imaging and Morphology (IM) Core is to meet the needs for microscopy imaging of the U-54 and NICHD-supported projects by providing superior facilities, expertise, assistance and training in the use of morphological methods and imaging technology. The Morphology component of the core will support U-54 scientists in paraffin tissue processing, sectioning, cryostat sectioning, histological, immunostaining and in situ hybridization. The Imaging component will offer digital image acquisition, color brightfield, dark field and fluorescence widefield, confocal imaging, image processing and analysis and procurement of pure populations of cells by laser capture microdissection. In support of approved projects, the Imaging & Morphology Core will: ¿ Provide equipment and technical assistance for the acquisition of frozen tissue sections using both cryostat and sliding microtomes. The Core will also make available space for storage of frozen tissue samples (-85¿C) and slide-mounted sections until they can be processed for histochemistry. ¿ Supply detailed protocols for immunohJstochemical (both double fluorescence and avidin-biotin complex [ABC] staining) and in situ hybridization (using both non- and isotopically-labeled cRNA probes) histochemical techniques, as well as assist in the optimization of procedures for specific applications. ,, Provide access to a 4-wavelength confocal microscope, equipped with high numerical aperture objectives, for acquisition of high-quality confocal images with exceptional z-axis resolution. ¿ Provide access to a laser capture microdissection system for the isolation of pure populations of cells for gene expression analysis and proteomics. ¿ Provide facilities for image processing and analysis. Although the majority of image processing and analysis occurs offline on desktop systems in investigator's laboratories, the Core maintains three workstations that are available for periodic use by laboratories with needs that do not justify purchase of their own system. ¿ Provide training for investigators in confocal microscopy, digital image capture, processing and analysis and laser microdissection. Assistance is also provided with specific experimental applications. Dr. Cornea will provide training in confocal microscopy and image processing. She is also available to consult on image acquisition and analysis strategies, as well as to assist in specific imaging applications and the development of analysis routines.

成像和形态学（IM）核心的目标是满足显微镜的需求 通过提供上级设施，专业知识， 在使用形态学方法和成像技术方面提供援助和培训。的 核心的形态组成部分将支持U-54科学家进行石蜡组织处理， 切片、低温恒温器切片、组织学、免疫染色和原位杂交。成像 组件将提供数字图像采集，彩色明场，暗场和荧光宽场， 共聚焦成像、图像处理和分析以及通过以下步骤获得纯细胞群： 激光捕获显微切割为了支持已批准的项目，成像和形态学核心 将： 为获取冷冻组织切片提供设备和技术援助， 低温恒温器和滑动切片机。核心还将提供存储空间， 冷冻组织样本（-85 ° C）和载玻片切片，直到它们可以被处理， 组织化学 提供详细的免疫组化方案（双荧光和抗生物素蛋白-生物素 复合物[ABC]染色）和原位杂交（使用非标记和同位素标记的cRNA 探针）组织化学技术，以及协助优化程序的具体 应用. 提供4波长共聚焦显微镜，配备高数值孔径 物镜，用于采集具有出色z轴分辨率的高质量共焦图像。 提供激光捕获显微切割系统，用于分离纯种群， 用于基因表达分析和蛋白质组学的细胞。 提供图像处理和分析设施。虽然大多数图像处理 核心认为，分析是在研究人员实验室的桌面系统上离线进行的 三个工作站可供实验室定期使用， 购买自己的系统。 为研究人员提供共聚焦显微镜、数字图像采集、处理和 分析和激光显微切割。还提供具体的实验援助， 应用. Cornea博士将提供共聚焦显微镜和图像处理方面的培训。她 也可以咨询图像采集和分析策略，并协助 特定的成像应用和分析程序的开发。